PXD011056

PXD011056 is an original dataset announced via ProteomeXchange.

Title	The response of Haloferax volcanii to salt and temperature stress: a proteome study by label-free mass spectrometry
Description	We performed an in-depth proteome analysis of the haloarchaeal model organism Haloferax volcanii under standard, low and high salt and low and high temperature conditions using label-free mass spectrometry. Qualitative analysis of protein identification data from high pH/reversed phase fractionated samples indicated 61.1% proteome coverage (2,509 proteins), which is close to the maximum recorded values in archaea. Identified proteins matched to the predicted proteome in their physicochemical protein properties, with only a small bias against low molecular wright and membrane-associated proteins. Cells grown under low and high salt stress as well as low and high temperature stress were quantitatively compared to standard cultures by SWATH mass spectrometry. 2,244 proteins, or 54.7% of the predicted proteome, were quantified across all conditions at high reproducibility which allowed for global analysis of protein expression changes under these stresses. Pathway enrichment analysis by KEGG annotation showed that most major cellular pathways are part of H. volcanii’s universal stress response. In addition, specific pathways were found to be selectively affected by either salt or temperature stress.
HostingRepository	PRIDE
AnnounceDate	2019-10-10
AnnouncementXML	Submission_2020-07-01_22:33:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-11 03:17:50	ID requested
1	2019-09-04 00:32:16	announced
2	2019-10-10 08:52:36	announced	2019-10-10: Updated project metadata.
⏵ 3	2020-07-01 22:33:14	announced	2019-10-10: Updated project metadata. 2020-07-02: Updated publication reference for PubMed record(s): 32561711, 31502396.

Schulze S, Adams Z, Cerletti M, De Castro R, Ferreira-Cerca S, Fufezan C, Gim, é, nez MI, Hippler M, Jevtic Z, Kn, ü, ppel R, Legerme G, Lenz C, Marchfelder A, Maupin-Furlow J, Paggi RA, Pfeiffer F, Poetsch A, Urlaub H, Pohlschroder M, The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics. Nat Commun, 11(1):3145(2020) [pubmed]

Jevti, ć Ž, Stoll B, Pfeiffer F, Sharma K, Urlaub H, Marchfelder A, Lenz C, The Response of Haloferax volcanii to Salt and Temperature Stress: A Proteome Study by Label-Free Mass Spectrometry. Proteomics, 19(20):e1800491(2019) [pubmed]

curator keyword: Biological

submitter keyword: Haloferax volcanii, archaea, proteome, SWATH, stress response, mass spectrometry

Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry; University Medical Center Goettingen
contact email	christof.lenz@mpibpc.mpg.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD011056

PRIDE project URI

• PRIDE
  1. PXD011056
     1. Label: PRIDE project
     2. Name: The response of Haloferax volcanii to salt and temperature stress: a proteome study by label-free mass spectrometry