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PXD011055

PXD011055 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAATF/Che-1 binds ribosome-associated RNAs and regulates ribosome biogenesis
DescriptionAATF is a predominantly nuclear protein that has been shown to be an apoptosis-inhibiting factor essential for both embryonic development and tumor growth. Several studies have shown a role of AATF in the modulation of pivotal cellular signal transduction pathways such as p53-, mTOR- and HIF-signaling. However, the exact molecular functions underlying its essential nature to cell proliferation and survival have remained elusive. Interestingly, several lines of evidence point towards a pivotal role of this protein in ribosome biogenesis and the maturation of ribosomal RNA. The function of AATF in this process is not clear, especially since the identity of the RNA-binding protein that links the AATF-containing protein complex (ANN) to rRNA precursors is unknown. In this study, we identify AATF in a screen for RNA-binding proteins. Importantly, AATF does not only bind to polyA-tailed RNA but CLIP-experiments reveal its association with ribosomal RNA. The RNA-binding domain of AATF is essential to maintain its positive effect on cellular rRNA levels. Furthermore, AATF binds to both mRNAs encoding for ribosome biogenesis factors as well as proteins involved with this process indicating AATF to be a central hub for the coordination of ribosome maturation. Consequently, our data for the first time identify the molecular basis to the role of AATF in ribosome biosynthesis and corroborate the link between tumorigenesis and ribosome biology.
HostingRepositoryPRIDE
AnnounceDate2019-08-13
AnnouncementXMLSubmission_2019-08-13_05:12:18.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Frese
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-11 00:10:57ID requested
12019-08-13 05:12:20announced
Publication List
Kaiser RWJ, Ignarski M, Van Nostrand EL, Frese CK, Jain M, Cukoski S, Heinen H, Schaechter M, Seufert L, Bunte K, Frommolt P, Keller P, Helm M, Bohl K, Höhne M, Schermer B, Benzing T, Höpker K, Dieterich C, Yeo GW, Müller RU, Fabretti F, A protein-RNA interaction atlas of the ribosome biogenesis factor AATF. Sci Rep, 9(1):11071(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: AATF
Contact List
Christian Frese
contact affiliationMax Planck Unit for the Science of Pathogens
contact emailfrese@mpiib-berlin.mpg.de
lab head
Christian Frese
contact affiliationMax Planck Unit for the Science of Pathogens
contact emailfrese@mpiib-berlin.mpg.de
dataset submitter
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