PXD011055

PXD011055 is an original dataset announced via ProteomeXchange.

Title	AATF/Che-1 binds ribosome-associated RNAs and regulates ribosome biogenesis
Description	AATF is a predominantly nuclear protein that has been shown to be an apoptosis-inhibiting factor essential for both embryonic development and tumor growth. Several studies have shown a role of AATF in the modulation of pivotal cellular signal transduction pathways such as p53-, mTOR- and HIF-signaling. However, the exact molecular functions underlying its essential nature to cell proliferation and survival have remained elusive. Interestingly, several lines of evidence point towards a pivotal role of this protein in ribosome biogenesis and the maturation of ribosomal RNA. The function of AATF in this process is not clear, especially since the identity of the RNA-binding protein that links the AATF-containing protein complex (ANN) to rRNA precursors is unknown. In this study, we identify AATF in a screen for RNA-binding proteins. Importantly, AATF does not only bind to polyA-tailed RNA but CLIP-experiments reveal its association with ribosomal RNA. The RNA-binding domain of AATF is essential to maintain its positive effect on cellular rRNA levels. Furthermore, AATF binds to both mRNAs encoding for ribosome biogenesis factors as well as proteins involved with this process indicating AATF to be a central hub for the coordination of ribosome maturation. Consequently, our data for the first time identify the molecular basis to the role of AATF in ribosome biosynthesis and corroborate the link between tumorigenesis and ribosome biology.
HostingRepository	PRIDE
AnnounceDate	2019-08-13
AnnouncementXML	Submission_2019-08-13_05:12:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Frese
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-11 00:10:57	ID requested
⏵ 1	2019-08-13 05:12:20	announced

Kaiser RWJ, Ignarski M, Van Nostrand EL, Frese CK, Jain M, Cukoski S, Heinen H, Schaechter M, Seufert L, Bunte K, Frommolt P, Keller P, Helm M, Bohl K, H, ö, hne M, Schermer B, Benzing T, H, ö, pker K, Dieterich C, Yeo GW, M, ü, ller RU, Fabretti F, A protein-RNA interaction atlas of the ribosome biogenesis factor AATF. Sci Rep, 9(1):11071(2019) [pubmed]

curator keyword: Biological

submitter keyword: AATF

Christian Frese
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	frese@mpiib-berlin.mpg.de
lab head
Christian Frese
contact affiliation	Max Planck Unit for the Science of Pathogens
contact email	frese@mpiib-berlin.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD011055

PRIDE project URI

• PRIDE
  1. PXD011055
     1. Label: PRIDE project
     2. Name: AATF/Che-1 binds ribosome-associated RNAs and regulates ribosome biogenesis