PXD011053

PXD011053 is an original dataset announced via ProteomeXchange.

Title	Proteoform-SRM (Semba et al. 2017)
Description	Circulating polypeptides and proteins have been implicated in reversing or accelerating aging phenotypes, including growth/differentiation factor 8 (GDF8), GDF11, eotaxin, and oxytocin. These proteoforms, which are defined as the protein products arising from a single gene due to alternative splicing and post-translational modifications, have been challenging to study. Both GDF8 and GDF11 have known antagonists such as follistatin (FST), and WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing proteins 1 and 2 (WFIKKN1, WFIKKN2). We developed a novel multiplexed selected reaction monitoring (SRM) assay using liquid chromatography-tandem mass spectrometry to measure five proteins related to GDF8 and GDF11 signaling, and in addition, eotaxin and oxytocin. Nineteen peptides consisting of 57 transitions were monitored and validated in pooled human plasma. In twenty-four adults, the mean (SD) concentrations (ng/mL) were as follows: GDF8 propeptide, 110.5 (24.2); GDF8 mature protein, 256.8 (80.4); GDF11 propeptide, 213.1 (108.9); GDF11 mature protein, 165.4 (124.1); FST, 298.1 (71.4); FST cleavage form FST303, 964.1 (691.6); WFIKKN1, 382.6 (82.9); WFIKKN2, 321.9 (105.0); oxytocin, 18.7 (9.5); and eotaxin, 23.4 (5.5). This novel multiplexed SRM assay should facilitate the study of the relationships of these proteoforms with major aging phenotypes.
HostingRepository	PeptideAtlas
AnnounceDate	2018-09-10
AnnouncementXML	Submission_2018-09-10_10:50:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pingbo Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine; S-carboxamidomethyl-L-cysteine
Instrument	QTRAP 5500

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-10 10:45:20	ID requested
⏵ 1	2018-09-10 10:50:13	announced

Semba RD, Zhang P, Zhu M, Fabbri E, Gonzalez-Freire M, Moaddel R, Geng-Spyropoulos M, Ferrucci L, A targeted proteomic assay for the measurement of plasma proteoforms related to human aging phenotypes. Proteomics, 17(15-16):(2017) [pubmed]

curator keyword: selected reaction monitoring, SRM, targeted

Pingbo Zhang
contact affiliation	Johns Hopkins University School of Medicine
contact email	pzhang7@jhmi.edu
dataset submitter
Richard D. Semba
contact affiliation	Wilmer Eye Institute, Johns Hopkins University School of Medicine
contact email	rdsemba@jhmi.edu
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI