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PXD011053

PXD011053 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteoform-SRM (Semba et al. 2017)
DescriptionCirculating polypeptides and proteins have been implicated in reversing or accelerating aging phenotypes, including growth/differentiation factor 8 (GDF8), GDF11, eotaxin, and oxytocin. These proteoforms, which are defined as the protein products arising from a single gene due to alternative splicing and post-translational modifications, have been challenging to study. Both GDF8 and GDF11 have known antagonists such as follistatin (FST), and WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing proteins 1 and 2 (WFIKKN1, WFIKKN2). We developed a novel multiplexed selected reaction monitoring (SRM) assay using liquid chromatography-tandem mass spectrometry to measure five proteins related to GDF8 and GDF11 signaling, and in addition, eotaxin and oxytocin. Nineteen peptides consisting of 57 transitions were monitored and validated in pooled human plasma. In twenty-four adults, the mean (SD) concentrations (ng/mL) were as follows: GDF8 propeptide, 110.5 (24.2); GDF8 mature protein, 256.8 (80.4); GDF11 propeptide, 213.1 (108.9); GDF11 mature protein, 165.4 (124.1); FST, 298.1 (71.4); FST cleavage form FST303, 964.1 (691.6); WFIKKN1, 382.6 (82.9); WFIKKN2, 321.9 (105.0); oxytocin, 18.7 (9.5); and eotaxin, 23.4 (5.5). This novel multiplexed SRM assay should facilitate the study of the relationships of these proteoforms with major aging phenotypes.
HostingRepositoryPeptideAtlas
AnnounceDate2018-09-10
AnnouncementXMLSubmission_2018-09-10_10:50:12.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPingbo Zhang
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine; S-carboxamidomethyl-L-cysteine
InstrumentQTRAP 5500
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-10 10:45:20ID requested
12018-09-10 10:50:13announced
Publication List
Semba RD, Zhang P, Zhu M, Fabbri E, Gonzalez-Freire M, Moaddel R, Geng-Spyropoulos M, Ferrucci L, A targeted proteomic assay for the measurement of plasma proteoforms related to human aging phenotypes. Proteomics, 17(15-16):(2017) [pubmed]
Keyword List
curator keyword: selected reaction monitoring, SRM, targeted
Contact List
Pingbo Zhang
contact affiliationJohns Hopkins University School of Medicine
contact emailpzhang7@jhmi.edu
dataset submitter
Richard D. Semba
contact affiliationWilmer Eye Institute, Johns Hopkins University School of Medicine
contact emailrdsemba@jhmi.edu
lab head
Full Dataset Link List
PeptideAtlas dataset URI
PASSEL experiment URI
PASSEL transition group browser URI