PXD011042

PXD011042 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of Burkholderia thailandensis during host infection
Description	Burkholderia pseudomallei and B. mallei are the causative agents of melioidosis and glanders, respectively, and are often fatal to humans and animals. Owing to the high fatality rate, potential for spread by aerosolization, and the lack of efficacious therapeutics, B. pseudomallei and B. mallei are considered biothreat agents of concern. In this study, we investigate the proteome of Burkholderia thailandensis, a closely related surrogate for the two more virulent Burkholderia species, during infection of host cells, and compare to that of B. thailandensis in culture. Studying the proteome of intracellular Burkholderia spp. is expected to reveal molecular mechanisms of intracellular survival and host immune evasion; but proteomic profiling of intracellular Burkholderia is challenging. Proteomic analyses of intracellular bacteria are typically hindered by the overwhelming host protein content recovered from infected cultures. To address this problem, we have applied bio-orthogonal noncanonical amino acid tagging (BONCAT) to B. thailandensis, enabling the enrichment of newly expressed bacterial proteins from virtually any growth condition, most importantly intracellular contexts. In this study, we show that B. thailandensis proteins were selectively labeled and efficiently enriched from infected host cells using BONCAT. We also demonstrate that this method can be used to label bacteria in situ by fluorescent tagging. Finally, we present a global proteomic profile of B. thailandensis as it infects host cells and a list of proteins that are differentially regulated in infection conditions as compared to bacterial monoculture. Among the identified proteins are quorum sensing regulated genes as well as homologs to previously identified virulence factors. This method provides a powerful tool to study the molecular processes during Burkholderia infection, a much-needed addition to the Burkholderia molecular toolbox.
HostingRepository	PRIDE
AnnounceDate	2018-11-12
AnnouncementXML	Submission_2018-11-12_09:11:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD011042
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Magdalena Franco
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Burkholderia thailandensis; NCBI TaxID: 57975;
ModificationList	Deamidated; Carbamidomethyl; Gln->pyro-Glu
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-10 02:49:33	ID requested
⏵ 1	2018-11-12 09:11:13	announced

Franco M, D'haeseleer PM, Branda SS, Liou MJ, Haider Y, Segelke BW, El-Etr SH, During Host Infection Using Bio-Orthogonal Noncanonical Amino Acid Tagging (BONCAT). Front Cell Infect Microbiol, 8():370(2018) [pubmed]

submitter keyword: Burkholderia thailandensis, LC-MS/MS, BONCAT

Magdelena Franco
contact affiliation	Lawrence Livermore National Laboratory
contact email	franco14@llnl.gov
lab head
Magdalena Franco
contact affiliation	Lawrence Livermore National Laboratory
contact email	franco14@llnl.gov
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD011042
     1. Label: PRIDE project
     2. Name: Proteomic profiling of Burkholderia thailandensis during host infection