PXD011032

PXD011032 is an original dataset announced via ProteomeXchange.

Title	SILAC-labeled proteins in ribosome samples
Description	we developed a novel method combining axon-pSILAC17 with axonal ribosome purification. In this method, we first labeled newly synthesized proteins with heavy amino acids (AAs) in somaless axons cultured in a Boyden chamber. Approximately 2000 eyes were cultured for each experiment to obtain sufficient axonal material. Then, axonal ribosomes were purified using a sucrose cushion and ultracentrifugation, followed by identification of newly synthesized proteins with mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2019-12-09
AnnouncementXML	Submission_2019-12-09_04:09:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Toshiaki Shigeoka
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	6x(13)C labeled residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-07 06:01:43	ID requested
⏵ 1	2019-12-09 04:09:54	announced

Dataset with its publication pending

submitter keyword: SILAC, Xenopus laevis, axon, ribosome

Christine E. Holt
contact affiliation	Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK.
contact email	ceh33@cam.ac.uk
lab head
Toshiaki Shigeoka
contact affiliation	University of Cambridge
contact email	ts561@cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD011032

PRIDE project URI

• PRIDE
  1. PXD011032
     1. Label: PRIDE project
     2. Name: SILAC-labeled proteins in ribosome samples