PXD011012

PXD011012 is an original dataset announced via ProteomeXchange.

Title	Quantitative (Glyco-)Proteomics of Haloferax volcanii Biofilm Formation
Description	A quantitative proteomics analysis comparing planktonic and sessile cells at different stages of biofilm development, using iTRAQ labeling, has been performed for Haloferax volcanii. Samples were taken from liquid, shaking cultures were compared to samples from static cultures, separated into sessile (biofilm) and planktonic cells. This dataset resulted in the identification of more than 50% of the H. volcanii proteome. Most importantly, expression as well as N-glycosylation of various surface filaments (pili, flagella and SLG) has been quantified, strongly supporting the hypothesis, that differential N-glycosylation plays important roles in the biofilm formation of H. volcanii.
HostingRepository	PRIDE
AnnounceDate	2020-05-11
AnnouncementXML	Submission_2020-07-01_22:31:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-05 06:49:28	ID requested
1	2020-05-10 22:45:21	announced
⏵ 2	2020-07-01 22:32:00	announced	2020-07-02: Updated publication reference for PubMed record(s): 32561711.

Schulze S, Adams Z, Cerletti M, De Castro R, Ferreira-Cerca S, Fufezan C, Gim, é, nez MI, Hippler M, Jevtic Z, Kn, ü, ppel R, Legerme G, Lenz C, Marchfelder A, Maupin-Furlow J, Paggi RA, Pfeiffer F, Poetsch A, Urlaub H, Pohlschroder M, The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics. Nat Commun, 11(1):3145(2020) [pubmed]

curator keyword: Biological

submitter keyword: Haloferax volcanii, Biofilm, Glycosylation, iTRAQ

Mechthild Pohlschroder
contact affiliation	Department of Biology, University of Pennsylvania
contact email	pohlschr@sas.upenn.edu
lab head
Stefan Schulze
contact affiliation	University of Pennsylvania
contact email	sschulze@sas.upenn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD011012

PRIDE project URI

• PRIDE
  1. PXD011012
     1. Label: PRIDE project
     2. Name: Quantitative (Glyco-)Proteomics of Haloferax volcanii Biofilm Formation