PXD011001

PXD011001 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic profiling reveals a defined genetic program for osteoblastic lineage commitment of human bone marrow derived stromal stem cells
Description	Bone marrow-derived mesenchymal stem cells (MSCs) differentiate into osteoblasts upon induction by signals present in their niche. As the global signaling cascades involved in the early phases of MSCs osteoblast (OB) differentiation are not well-defined, we employed quantitative mass spectrometry (SILAC based) to delineate changes in human MSCs proteome and phosphoproteome during the first 24 hours of their OB lineage commitment. The temporal profiles of 6,252 proteins and 15,059 phosphorylation sites suggested at least two distinct signaling waves: one peaking within 30 to 60 min after induction and a second upsurge after 24 hours
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_07:47:19.036.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Inigo Barrio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-04 02:34:36	ID requested
⏵ 1	2021-09-08 07:47:19	announced

Barrio-Hernandez I, Jafari A, Rigbolt KTG, Hallenborg P, Sanchez-Quiles V, Skovrind I, Akimov V, Kratchmarova I, Dengjel J, Kassem M, Blagoev B, Phosphoproteomic profiling reveals a defined genetic program for osteoblastic lineage commitment of human bone marrow-derived stromal stem cells. Genome Res, 30(1):127-137(2020) [pubmed]

curator keyword: Biological

submitter keyword: Osteoblast, MSCs, SILAC, differentiation, mass spectrometry, phosphorylation

Blagoy Blagoev
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark
contact email	bab@bmb.sdu.dk
lab head
Inigo Barrio
contact affiliation	EMBL-EBI
contact email	ibarrioh@ebi.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD011001

PRIDE project URI

• PRIDE
  1. PXD011001
     1. Label: PRIDE project
     2. Name: Phosphoproteomic profiling reveals a defined genetic program for osteoblastic lineage commitment of human bone marrow derived stromal stem cells