PXD010999

PXD010999 is an original dataset announced via ProteomeXchange.

Title	A novel truncated form of Nephronectin is present in small extracellular vesicles isolated from 66cl4-cells
Description	Extracellular vesicles are quickly emerging as biomarkers in breast cancer. Our recent report suggested that an intracellular granular staining pattern of the extracellular matrix protein Nephronectin (NPNT) in breast tumor sections correlated with poor prognosis. In this study we performed proteomic analysis which reveals that overexpression of NPNT in breast cancer cells alters the cargo of its NPNT-containing small extracellular vesicles, and includes several tumor promoting proteins. We identify three different forms of NPNT at 80, 60 and 20 kDa. We report that the native form of NPNT at 60 kDa gets further glycosylated and is detected as the 80 kDa NPNT, which maybe truncated then by matrix metalloproteinases to a shorter form of around 20 kDa. Although both 80 kDa and 20 kDa NPNT are detected in small extracellular vesicles (sEVs) derived from breast cancer cells, the truncated form of NPNT is concentrated in sEVs, which is a novel finding.
HostingRepository	PRIDE
AnnounceDate	2019-02-15
AnnouncementXML	Submission_2019-02-15_08:41:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-04 02:25:51	ID requested
⏵ 1	2019-02-15 08:41:08	announced

Toraskar J, Magnussen SN, Hagen L, Sharma A, Hoang L, Bj, ø, rk, ø, y G, Svineng G, Steigedal TS, A Novel Truncated Form of Nephronectin Is Present in Small Extracellular Vesicles Isolated from 66cl4 Cells. J Proteome Res, 18(3):1237-1247(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Breast Cancer, Nephronectin, Small Extracellular Vesicles, MaxLFQ, proteome

Lars Hagen
contact affiliation	Proteomics and Modomics Experimental Core Facility (PROMEC) Department of Clinical and Molecular Medicine Medisinsk teknisk forskningssenter Olav Kyrres gt. 9 N-7489 Trondheim Norway
contact email	lars.hagen@ntnu.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD010999

PRIDE project URI

• PRIDE
  1. PXD010999
     1. Label: PRIDE project
     2. Name: A novel truncated form of Nephronectin is present in small extracellular vesicles isolated from 66cl4-cells