PXD010998

PXD010998 is an original dataset announced via ProteomeXchange.

Title	larval integument of silkworm LC-MSMS
Description	The Dominant obese translucent (Obs) mutant of the silkworm results in a short and stout larval body, translucent phenotype, and abnormal pigmentation in the integument. The Obs mutant also displays difficulties in ecdysis and metamorphosis. In the present study, to gain an understanding of multiple Obs phenotypes, we investigated the phenotypes of Obs and performed a comparative analysis of the larval integument proteomes of Obs and normal silkworms. The phenotypic analysis revealed that the Obs larvae are indeed short and fat, and that chitin and uric acid content were lower but melanin content was higher in the Obs mutant. Proteomic analysis revealed that 244 proteins were significantly differentially expressed between Obs and normal silkworms, some of which are involved in uric acid metabolism and melanin pigmentation. Twenty-six proteins were annotated as cuticular proteins, including RR motif-rich cuticular proteins (CPR), glycine-rich cuticular protein (CPG), hypothetical cuticular protein (CPH), cuticular protein analogous to peritrophins (CPAP), and the chitin_bind_3 motif proteins, and accounted for over 84% of the abundance of the total significantly differentially-expressed proteins identified. Moreover, 22 of the 26 cuticular proteins were down-regulated in the Obs mutant. Comparative proteomic analysis suggested that the multiple phenotypes of the Obs mutant are related to changes in the expression of proteins that participate in cuticular formation, uric acid metabolism, and melanin pigmentation. These results also provide a reference for studying the gene responsible for the Obs mutant.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:07:01.224.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	kun wang
SpeciesList	scientific name: Bombyx mori (Silk moth); NCBI TaxID: 7091;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-04 02:25:07	ID requested
1	2018-11-15 14:12:45	announced
⏵ 2	2024-10-22 04:07:02	announced	2024-10-22: Updated project metadata.

Wang L, Dong Z, Wang J, Yin Y, Liu H, Hu W, Peng Z, Liu C, Li M, Banno Y, Shimada T, Xia Q, Zhao P, Proteomic Analysis of Larval Integument in a Dominant Obese Translucent (Obs) Silkworm Mutant. J Insect Sci, 18(6):(2018) [pubmed]

10.1093/jisesa/iey098;

curator keyword: Biological

submitter keyword: LC-MSMS,silkworm,larval integument

Qingyou Xia
contact affiliation	State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing , China.
contact email	xiaqy@swu.end.cn
lab head
kun wang
contact affiliation	13032305499
contact email	katrinawangk@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010998
     1. Label: PRIDE project
     2. Name: larval integument of silkworm LC-MSMS