We sought to characterize the proteome and metabolome of S. vermifera and S. bescii under N and P starvation conditions in vitro. As very little is known about the ecological functioning of the Serendipitaceae in most environments, the overall objective of the study was to elucidate the cellular response(s) of two Serendipitaceae strains toward stress imposed by the lack of one or the other of these important nutrients. Our approach allows us to not only evaluate N and P acquisition processes in the understudied Serendipitaceae, but also to understand the physiological responses to their restriction.