PXD010993

PXD010993 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SWATH proteomic profiling of prostate cancer cells identifies NUSAP1 as a biomarker of response to Galiellalactone
Description	Galiellalactone (GL) is a fungal metabolite that presents antitumor and anti-inflammatory activities in vitro and in vivo. Previous studies have shown that GL targets NF-KB and STAT3 pathways and induces G2/M cell cycle arrest in androgen-insensitive prostate cancer cells. In this study, we show that GL-induced cell cycle arrest is independent of the NF-KB and STAT3 pathways in DU145 and PC-3 cells, and also that GL did not affect cell cycling in androgen-sensitive prostate cancer cell such as LNCaP and 22Rv1 cells. In addition, we showed confluence resistance to GL in DU145 cells. Using a SWATH proteomic approach we identified the down-regulation of Nucleolar and spindle associated protein 1 (NUSAP1) under DU145 confluence and in LNCaP cells. Also, the inhibition of NUSAP1 by siRNAs induced resistance to GL in DU145 cells, suggesting that NUSAP1 may be a target for GL and could be useful as biomarker for responsiveness of the antitumor activity of GL.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:56.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignacio Ortea
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-03 05:42:09	ID requested
1	2019-01-14 04:39:20	announced
⏵ 2	2024-10-22 04:06:58	announced	2024-10-22: Updated project metadata.

Publication List

Garrido-Rodr, í, guez M, Ortea I, Calzado MA, Mu, ñ, oz E, Garc, í, a V, SWATH proteomic profiling of prostate cancer cells identifies NUSAP1 as a potential molecular target for Galiellalactone. J Proteomics, 193():217-229(2019) [pubmed]
10.1016/j.jprot.2018.10.012;

Garrido-Rodr, í, guez M, Ortea I, Calzado MA, Mu, ñ, oz E, Garc, í, a V, SWATH proteomic profiling of prostate cancer cells identifies NUSAP1 as a potential molecular target for Galiellalactone. J Proteomics, 193():217-229(2019) [pubmed]

10.1016/j.jprot.2018.10.012;

Keyword List

curator keyword: Biomedical
submitter keyword: Galiellalactone
cell cycle
prostate cancer
SWATH
NUSAP1

curator keyword: Biomedical

submitter keyword: Galiellalactone

cell cycle

prostate cancer

SWATH

NUSAP1

Contact List

Ignacio Ortea
contact affiliation	Proteomics Unit, IMIBIC, Cordoba, Spain
contact email	ignacio.ortea@imibic.org
lab head
Ignacio Ortea
contact affiliation	IMIBIC
contact email	iortea@gmail.com
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010993

PRIDE project URI

Repository Record List

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