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PXD010989

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2019-03-14
  • AnnouncementXML: Submission_2019-03-21_09:48:10.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Jennifer Jung
  • Title: TAPL interaction network
  • Description: The human lysosomal polypeptide ABC transporter TAPL (ABC subfamily B member 9, ABCB9) transports 6–59 amino-acids-long polypeptides from the cytosol into lysosomes. The subcellular localization of TAPL depends solely on its N-terminal transmembrane domain TMD0, which lacks conventional targeting sequences. However, the intracellular route and the molecular mechanisms that control TAPL localization remain unclear. Here, we delineated the route of TAPL to lysosomes and investigated the determinants of single trafficking steps. By synchronizing trafficking events by retention using selective hooks (RUSH) assay and visualizing individual intermediate steps through immunostaining and confocal microscopy, we demonstrate that TAPL takes the direct route to lysosomes. We further identified conserved charged residues within TMD0 transmembrane helices that are essential for individual steps of lysosomal targeting. Substitutions of these residues retained TAPL in the endoplasmic reticulum (ER) or Golgi. We also observed that for release from the ER, a salt bridge between Asp-17 and Arg-57 is essential. An interactome analysis revealed that Yip1-interacting factor homolog B, membrane-trafficking protein (YIF1B) interacts with TAPL. We also found that YIF1B is involved in ER-to-Golgi trafficking and interacts with TMD0 of TAPL via its transmembrane domain and that this interaction strongly depends on the newly identified salt bridge within TMD0. These results expand our knowledge about lysosomal trafficking of TAPL and the general function of extra transmembrane domains of ABC transporters.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: No PTMs are included in the dataset
  • Instrument: LTQ Orbitrap Velos

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-09-03 03:41:28ID requested
12019-03-14 08:05:02announced
22019-03-21 09:48:11announcedUpdated publication reference for PubMed record(s): 30877195.

Publication List

  1. Graab P, Bock C, Weiss K, Hirth A, Koller N, Braner M, Jung J, Loehr F, Tampé R, Behrends C, Abele R, Lysosomal targeting of the ABC transporter TAPL is determined by membrane-localized charged residues. J Biol Chem, 294(18):7308-7323(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: LC-MS

Contact List

    Christian Behrends
    • contact affiliation: Munich Cluster for Systems Neurology, Ludwig-Maximilians-University, Feodor-Lynen Str. 17, 81377 München
    • contact email: Christian.Behrends@mail03.med.uni-muenchen.de
    • lab head:
    Jennifer Jung
    • contact affiliation: Scientist
    • contact email: jenneyjung@gmx.de
    • dataset submitter:

Full Dataset Link List

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  2. PRIDE project URI
Repository Record List
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