PXD010988

PXD010988 is an original dataset announced via ProteomeXchange.

Title	Thanatin Targets the Inter-Membrane Protein Complex Required for Lipopolysaccharide Transport in Escherichia coli
Description	With the increasing resistance of many Gram-negative bacteria to existing classes of antibiotics, identifying new paradigms in antimicrobial discovery is an important research priority. Of special interest here are the proteins required for the biogenesis of the symmetric Gram-negative bacterial outer membrane. Seven Lpt proteins (LptA-G) associate in most Gram-negative bacteria to form a macromolecular complex spanning the entire envelope, which transports LPS molecules from their site of assembly at the inner membrane to the cell surface, powered by ATP hydrolysis in the cytoplasm. The periplasmic protein LptA comprises the protein bridge across the periplasm, which connects LptB2FGC at the inner membrane to LptD/E anchored in the outer membrane. We show here that the naturally occurring insect derived antimicrobial peptide thanatin targets LptA and LptD in the network of periplasmic protein-protein interactions required to assemble the Lpt complex, leading to inhibition ofLPS transport and OM biogenesis in Escherichia coli.
HostingRepository	PRIDE
AnnounceDate	2019-01-23
AnnouncementXML	Submission_2019-01-23_00:43:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maik Müller
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-03 03:39:11	ID requested
⏵ 1	2019-01-23 00:43:45	announced

Vetterli SU, Zerbe K, M, ü, ller M, Urfer M, Mondal M, Wang SY, Moehle K, Zerbe O, Vitale A, Pessi G, Eberl L, Wollscheid B, Robinson JA, . Sci Adv, 4(11):eaau2634(2018) [pubmed]

curator keyword: Biological

submitter keyword: Gram-negative ESKAPE pathogens, E.coli, macrocyclic peptidomimetic antibiotics, OMPTA (outer membrane-targeting antibiotics), Lpt complex, photo crosslinking, photoaffinity labeling, interaction mapping, DDA, LC-MS/MS, Thermo Q-Exactive Plus, molecular mode of action

Bernd Wollscheid
contact affiliation	Head of Proteomics Plattform Department of Health Sciences and Technology Institute of Molecular Systems Biology ETH Zurich Switzerland
contact email	bernd.wollscheid@imsb.biol.ethz.ch
lab head
Maik Müller
contact affiliation	ETH Zurich
contact email	maimuell@ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010988

PRIDE project URI

• PRIDE
  1. PXD010988
     1. Label: PRIDE project
     2. Name: Thanatin Targets the Inter-Membrane Protein Complex Required for Lipopolysaccharide Transport in Escherichia coli