PXD010987

PXD010987 is an original dataset announced via ProteomeXchange.

Title	Smell of Infection: a novel, non-invasive method for detection of fish excretory- secretory proteins
Description	Chemical signals are produced by aquatic organisms following predatory attacks or perturbations such as parasitic infection. Ectoparasites feeding on host fish are likely to cause release of similar alarm cues into the environment due to the stress, wounding and immune response stimulated upon infection. Alarm cues are often released in the form of proteins and peptides and provide important insights into bodily function and infection status. Here we outline a non-invasive method to identify potential chemical cues associated with infection in fish by extracting, purifying and characterizing proteins from water samples from cultured fish. Gel free proteomic methods were deemed the most suitable for protein detection in saline water samples. It was confirmed that proteins could be characterized from teleost water samples and that variation in protein profiles could be detected between infected and uninfected individuals and fish and parasite only water samples. Our novel assay provides a non-invasive method for assessing the health condition of both wild and farmed aquatic organisms. Similar to environmental DNA monitoring methods, these proteomic techniques could provide an important tool in applied biology and aquaculture biology
HostingRepository	PRIDE
AnnounceDate	2019-01-02
AnnouncementXML	Submission_2019-01-02_02:22:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rebecca Pawluk
SpeciesList	scientific name: Kryptolebias marmoratus; NCBI TaxID: 37003;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6550 iFunnel Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-09-03 03:32:15	ID requested
⏵ 1	2019-01-02 02:22:26	announced

Pawluk RJ, Stuart R, Garcia de Leaniz C, Cable J, Morphew RM, Brophy PM, Consuegra S, Smell of Infection: A Novel, Noninvasive Method for Detection of Fish Excretory-Secretory Proteins. J Proteome Res, 18(3):1371-1379(2019) [pubmed]

curator keyword: Biological

submitter keyword: Alarm cues, gel free MS,parasitic infection

Prof Peter M Brophy
contact affiliation	Professor in Parasitology at Aberystwyth University
contact email	pmb@aber.ac.uk
lab head
Rebecca Pawluk
contact affiliation	Swansea Univeristy
contact email	rebeccajane93@hotmail.co.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010987
     1. Label: PRIDE project
     2. Name: Smell of Infection: a novel, non-invasive method for detection of fish excretory- secretory proteins