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PXD010986

PXD010986 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic profile of mouse hippocampus following kainate-induced epileptogenesis C4PR_LIV
DescriptionAnti-epileptogenic agents that prevent the development of epilepsy following a brain insult remain the holy grail of epilepsy therapeutics. In order to identify such drugs, it is necessary to first understand the cellular and molecular events that underlie the epileptogenic process, from initial insult to the onset of spontaneous recurrent seizures. We have employed a label-free proteomic approach that allows quantification of large numbers of brain-expressed proteins in a single analysis in the well-established kainate (KA) mouse (C57BL/6J) model of epileptogenesis. In addition, we have incorporated two putative antiepileptogenic drugs, PSD95BP and 1400W, to give an insight into how such agents might ameliorate the epileptogenesis. The test drugs were administered at appropriate time-points after induction of status epilepticus (SE) and animals were euthanized at 7 days, their hippocampi removed, and subjected to LC-MS/MS analysis. A total of 2,579 proteins were identified; their normalized abundance was compared between treatment groups using ANOVA, with correction for multiple testing by false discovery rate. Significantly altered proteins were subjected to gene ontology analysis to look for enrichment of specific biological processes. KA-induced SE was most robustly associated with an increase in proteins involved in neuroinflammation, oxidative stress, cell-cell interactions and synaptic plasticity. Treatment with PSD95BP (Tat-NR2B9c) or an iNOS inhibitor, 1400W modulated several of these proteins. Our observations require validation in a larger-scale investigation, with candidate proteins explored in more detail. Nevertheless, this study has identified several mechanisms by which epilepsy might be developed and several targets for novel drug development.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:07:14.701.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD010986
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDeborah Simpson
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListOxidation; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-09-03 03:08:58ID requested
12019-05-23 01:37:27announced
22024-10-22 04:07:15announced2024-10-22: Updated project metadata.
Publication List
Tse K, Hammond D, Simpson D, Beynon RJ, Beamer E, Tymianski M, Salter MW, Sills GJ, Thippeswamy T, The impact of postsynaptic density 95 blocking peptide (Tat-NR2B9c) and an iNOS inhibitor (1400W) on proteomic profile of the hippocampus in C57BL/6J mouse model of kainate-induced epileptogenesis. J Neurosci Res, 97(11):1378-1392(2019) [pubmed]
10.6019/PXD010986;
10.1002/jnr.24441;
Keyword List
curator keyword: Biomedical
submitter keyword: LC-MSMS, Kainic acid,Mouse, Hippocampus, epileptogenesis
Contact List
Thimmasettappa Thippeswamy
contact affiliationEpilepsy Research Laboratory Department of Biomedical Sciences College of Veterinary Medicine Iowa State University Ames Iowa 50011-1250 USA
contact emailtswamy@iastate.edu
lab head
Deborah Simpson
contact affiliationUniversity of Liverpool
contact emaildsimpson@liverpool.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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