PXD010974

PXD010974 is an original dataset announced via ProteomeXchange.

Title	Study physiological and biochemical response of Phaeocystis antarctica to different irradiance and manganese/ iron deficiency
Description	Iron and light are typically recognized as major limiting factors controlling phytoplankton growth in the Southern Ocean. Recent field-based evidence suggests, however, that manganese concentrations in this region can be low enough to impact phytoplankton physiology and primary productivity. Our study examined the interactive influence of combined iron and manganese deprivation on protein expression and photophysiology in Phaeocystis antarctica, a key Antarctic phytoplankter, and provide taxon-specific proteomic evidence that natural Southern Ocean Phaeocystis populations regularly experience stress due to combined low manganese and iron availability. In culture, combined low iron and manganese induced large scale changes in the Phaeocystis proteome and resulted in reorganization of key components of the photosynthetic apparatus; these differences were largely distinct from those arising from changes in irradiance. These results implicate manganese availability as an important driver of Southern Ocean productivity and demonstrate the utility of peptide mass spectrometry as a tool for mapping of manganese contributions to HNLC conditions in this region.
HostingRepository	PRIDE
AnnounceDate	2019-06-25
AnnouncementXML	Submission_2019-06-25_01:09:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miao Wu
SpeciesList	scientific name: Phaeocystis antarctica; NCBI TaxID: 33657;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-31 06:07:28	ID requested
⏵ 1	2019-06-25 01:09:04	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Phaeocystis Antarctica, iron, manganese, deprivation, photosynthesis, proteomic

Erin M. Bertrand
contact affiliation	Department of Biology, Dalhousie University, Halifax Nova Scotia Canada B3H-4R2
contact email	erin.bertrand@dal.ca
lab head
Miao Wu
contact affiliation	Swedish University of Agricultural Sciences
contact email	miao.wu@slu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD010974

PRIDE project URI

• PRIDE
  1. PXD010974
     1. Label: PRIDE project
     2. Name: Study physiological and biochemical response of Phaeocystis antarctica to different irradiance and manganese/ iron deficiency