PXD010967

PXD010967 is an original dataset announced via ProteomeXchange.

Title	First viruses infecting the marine diatom Guinardia delicatula
Description	The marine diatom Guinardia delicatula is a cosmopolitan species that dominates seasonal blooms in the English Channel and the North Sea. Several eukaryotic parasites are known to induce the mortality of this key-stone species. Here, we report the isolation and the characterization of the first viruses that infect G. delicatula. Viruses were isolated from the Western English Channel (SOMLIT-ASTAN station) during the late summer bloom decline of G. delicatula. A combination of laboratory approaches revealed that these lytic viruses (GdelRNAV) are small untailed particles of 35-38 nm in diameter that replicated in the host cytoplasm where both unordered particles and crystalline arrays were formed. GdelRNAV displayed a linear single-stranded RNA genome of ~9 kb, including two open reading frames encoding for replication and structural polyproteins. Phylogenetic relationships based on the RNA-dependent-RNA-polymerase gene marker showed that GdelRNAV were new members of the Bacillarnavirus, a monophyletic genus belonging to the order Picornavirales. GdelRNAV were specific to several strains of G. delicatula, they were produced rapidly (< 12h) and in numbers (9.34 x 104 virions per host cell). We recorded a substantial delay (72 h) between virions release and host cell lysis. Our analysis points to variable viral susceptibilities of the host during the early exponential growth phase. Interestingly, we consistently failed to isolate viruses during spring and early summer while G. delicatula developed rapid and massive blooms. While our study suggests that viruses do contribute to the decline of G. delicatula late summer bloom, they may not be the primary mortality agents during the remaining blooms at SOMLIT-ASTAN. Future studies should focus on the relative contribution of the viral and eukaryotic pathogens to the control of Guinardia blooms to understand the fate of these prominent organisms in marine systems.
HostingRepository	PRIDE
AnnounceDate	2019-01-30
AnnouncementXML	Submission_2019-01-30_10:57:52.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010967
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	E Com
SpeciesList	scientific name: unclassified Bacillarnavirus; NCBI TaxID: 1522178;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-31 05:35:10	ID requested
⏵ 1	2019-01-30 10:57:53	announced

Arsenieff L, Simon N, Rigaut-Jalabert F, Le Gall F, Chaffron S, Corre E, Com E, Bigeard E, Baudoux AC, . Front Microbiol, 9():3235(2018) [pubmed]

curator keyword: Biological

submitter keyword: single-stranded RNA viruses, Diatoms, Genomics, Host-virus dynamics, Western English Channel

Charles Pineau
contact affiliation	Protim, Irset, Inserm U1085, Rennes, France
contact email	charles.pineau@inserm.fr
lab head
E Com
contact affiliation	Protim, Irset, Inserm U1085
contact email	emmanuelle.com@univ-rennes1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010967

PRIDE project URI

• PRIDE
  1. PXD010967
     1. Label: PRIDE project
     2. Name: First viruses infecting the marine diatom Guinardia delicatula