PXD010962

PXD010962 is an original dataset announced via ProteomeXchange.

Title	Naja annulifera Snake: New Insights into the Venom Components and Pathogenesis of Envenomation
Description	Naja annulifera is a dangerous snake that belongs to the Elapidae family. It is found in some of the countries in Sub-Saharan Africa and has caused several accidents in humans and dogs. In this study, we characterized some of the biochemical, toxic and immunogenic properties of N. annulifera venom. We showed that the venom is composed of several proteins, some of which display enzymatic activities, such as phospholipase A2, hyaluronidase, metalloproteinases and serine proteinases. The venom promoted disturbances in the human coagulation system and was cytotoxic to human epidermal cells. Using a mouse model, we showed that the venom promotes local reactions that were reduced with anti-inflammatory drugs. The venom caused systemic inflammation, lung hemorrhage and death. Further, the venom stimulated production of high antibody titers when injected into mice and the antiserum produced was able to inhibit venom-induced death. We therefore conclude that N. annulifera venom contains toxins that play a role in the toxic and inflammatory effects that contribute to the clinical manifestations and death. Moreover, the venom is immunogenic, an important aspect for the production an efficient N. annulifera antivenom.
HostingRepository	PRIDE
AnnounceDate	2019-02-25
AnnouncementXML	Submission_2019-02-25_07:27:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduardo Kitano
SpeciesList	scientific name: Naja annulifera; NCBI TaxID: 96794;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-31 02:17:16	ID requested
⏵ 1	2019-02-25 07:27:38	announced

Silva-de-Fran, ç, a F, Villas-Boas IM, Serrano SMT, Cogliati B, Chudzinski SAA, Lopes PH, Kitano ES, Okamoto CK, Tambourgi DV, Naja annulifera Snake: New insights into the venom components and pathogenesis of envenomation. PLoS Negl Trop Dis, 13(1):e0007017(2019) [pubmed]

curator keyword: Biological

submitter keyword: Naja annulifera snake venom, toxins, inflammation, antivenom, LC-MS/MS, iBAQ

Solange M. T. Serrano
contact affiliation	Special Laboratory of Applied Toxinology, Butantan Institute, São Paulo, Brazil
contact email	solange.serrano@butantan.gov.br
lab head
Eduardo Kitano
contact affiliation	Butantan Institute
contact email	eduardosh.kitano@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/02/PXD010962

PRIDE project URI

• PRIDE
  1. PXD010962
     1. Label: PRIDE project
     2. Name: Naja annulifera Snake: New Insights into the Venom Components and Pathogenesis of Envenomation