PXD010960

PXD010960 is an original dataset announced via ProteomeXchange.

Title	Porcine breast extracellular matrix hydrogel for spatial tissue culture
Description	Porcine mammary fatty tissues represent an abundant source of biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel scaffolds from the extracted ECM proteins, the structural properties of the scaffolds, and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.
HostingRepository	PRIDE
AnnounceDate	2019-11-12
AnnouncementXML	Submission_2019-11-12_13:37:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Wang
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics solarix series

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-31 02:16:00	ID requested
⏵ 1	2019-11-12 13:37:06	announced

Rijal G, Wang J, Yu I, Gang DR, Chen RK, Li W, Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture. Int J Mol Sci, 19(10):(2018) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP)

curator keyword: Biological

submitter keyword: porcine, breast cancer, ECM, hydrogel, scaffold, 3D culture

Weimin Li
contact affiliation	Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, WA 99210
contact email	weimin.li@wsu.edu
lab head
Jing Wang
contact affiliation	WSU
contact email	jing.wang6@wsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD010960

PRIDE project URI

• PRIDE
  1. PXD010960
     1. Label: PRIDE project
     2. Name: Porcine breast extracellular matrix hydrogel for spatial tissue culture