PXD010955

PXD010955 is an original dataset announced via ProteomeXchange.

Title	iTRAQ-based quantitative proteomic analysis of Takifugu fasciatus liver in response to low temperature stress
Description	The effects of temperature on teleosts have been of strong interest for a long time, particularly because low temperature profoundly influences the physiological and behavioral processes of ectotherms, especially teleosts. However, the cold-tolerance characteristics of fish at a protein level remains unclear. Therefore, in order to shed further light on the molecular mechanisms of low temperature adaptation in fish, we conducted quantitative proteomics on T. fasciatus liver using iTRAQ. Comparing the proteomic profiles of the T. fasciatus liver at 12℃ and 26℃, a total of 3741 proteins were identified, and 160 were differentially expressed. Among the differentially expressed proteins, the most significant changes were noted in those involved in oxidative stress (nine proteins), mitochondrial enzymes (eleven proteins) and signal transduction (thirteen proteins). The KEGG enrichment analysis indicated significant enhancement of D-arginine and D-ornithine metabolism, MAPK signaling, Wnt signaling and Gap junction pathway. Subsequently, three significantly up-regulated (CIRB, HSP90 and GST) and two significantly down-regulated proteins (FLNB and A2ML1) were validated by the parallel reaction monitoring (PRM) assays. Furthermore, the changes in expression of proteins involved in the oxidative stress, mitochondrial enzymes and signal transduction were validated at the transcriptional level using qPCR. These verification results show that the experimental data of iTRAQ are reliable. Our results not only deepen an understanding of the mechanisms underlying low temperature tolerance in fish, but also may contribute to its breeding for enhancement of cold tolerance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:07:36.899.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xin Wen
SpeciesList	scientific name: Takifugu fasciatus; NCBI TaxID: 301270;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-30 09:33:03	ID requested
1	2019-05-09 06:44:57	announced
⏵ 2	2024-10-22 04:07:37	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2019.04.004;

Wen X, Zhang X, Hu Y, Xu J, Wang T, Yin S, iTRAQ-based quantitative proteomic analysis of Takifugu fasciatus liver in response to low-temperature stress. J Proteomics, 201():27-36(2019) [pubmed]

ProteomeXchange project tag: Mitochondria (HPP)

curator keyword: Biological

submitter keyword: Environmental stress

Proteomics

Low temperature

iTRAQ

PRM

Takifugu fasciatus

Shaowu Yin
contact affiliation	College of Marine Science and Engineering, Nanjing Normal University, Nanjing 210023, China
contact email	yinshaowu@163.com
lab head
Xin Wen
contact affiliation	Hainan university
contact email	wenxinfish@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD010955

PRIDE project URI

• PRIDE
  1. PXD010955
     1. Label: PRIDE project
     2. Name: iTRAQ-based quantitative proteomic analysis of Takifugu fasciatus liver in response to low temperature stress