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PXD010948

PXD010948 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTo invesigate the role of autophagy in Arabidopsis shoot cellular protein turnover and proteomstasis (15N Spike-in shoot)
DescriptionProtein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lead to accumulation of protein targets with/without starvation stress. Further protein turnover analysis uncover a subgroup of proteins with retarded degradation. New selective autophagy protein targets involving glycolytic proteins were uncovered by this strategy. Furthermore, we found plasma membrane and cytoskeleton proteins show a general reduction, supporting the cross-interaction between autopahgy and vesicle trafficking pathway. Metabolomics changes in autophagy mutants due to a changed proteostasis was further investigated.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:20:34.777.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLei Li
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-30 07:07:56ID requested
12022-10-13 04:41:46announced
22023-11-14 07:20:35announced2023-11-14: Updated project metadata.
Publication List
Li L, Lee CP, Ding X, Qin Y, Wijerathna-Yapa A, Broda M, Otegui MS, Millar AH, Defects in autophagy lead to selective in vivo changes in turnover of cytosolic and organelle proteins in Arabidopsis. Plant Cell, 34(10):3936-3960(2022) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Arabidopsis, protein turnover, 15N spike-in, autophagy
Contact List
Harvey Millar
contact affiliationARC Centre of Excellence in Plant Energy Biology, M316, 35 Stirling Hwy, 6009, WA, Australia Faculty of Science, The University of Western Australia
contact emailharvey.millar@uwa.edu.au
lab head
Lei Li
contact affiliationCollege of Life Sciences, Nankai University, Tianjin 300071
contact emaillei.li@nankai.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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