⮝ Full datasets listing

PXD010942

PXD010942 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEnhanced RNA Interactome capture (eRIC) and RNA Interactome capture (RIC) of Jurkat cells (DSMZ, ACC-282) exposed to 0.5mM DMOG or vehicle (DMSO) for 6 hours.
Description1.0 – 1.3 x 10e8 proliferating Jurkat cells at a density of about 1-1.5 x 10e6 cells/mL were employed per sample. Cells were incubated with 0.5mM DMOG (Cayman Chemical Company, 71210) or an equivalent volume of Dimethyl Sulfoxide (DMSO) (vehicle, Merck 1.02950.0500). The DMSO concentration in the medium was 0.023% v/v. Cells were irradiated or not with 254 nm UV light and subjected to eRIC or RIC to determine the RNA-bound proteome. Data correspond to two biologically independent experiments.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:08:02.409.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrank Stein
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-30 02:30:30ID requested
12018-09-03 03:54:54announced
22018-09-11 00:35:02announcedUpdated project metadata.
32018-11-04 14:46:29announcedUpdated project metadata.
42024-10-22 04:08:03announced2024-10-22: Updated project metadata.
Publication List
Perez-Perri JI, Rogell B, Schwarzl T, Stein F, Zhou Y, Rettel M, Brosig A, Hentze MW, Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture. Nat Commun, 9(1):4408(2018) [pubmed]
10.1038/s41467-018-06557-8;
Keyword List
curator keyword: Biomedical
submitter keyword: DMOG, RIC, eRIC, N6-methyladenosine , epitranscriptome, m6A,RNA interactome, RBPs, RBPome, RNA-binding proteins, dimethyloxalylglycine
Contact List
Matthias W. Hentze
contact affiliationEuropean Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
contact emailhentze@embl.de
lab head
Frank Stein
contact affiliationEMBL
contact emailfrank.stein@embl.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/09/PXD010942
PRIDE project URI
Repository Record List
[ + ]