PXD010938

PXD010938 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Comparative proteomics using iodoTMT to identify changes in protein expression and cysteine oxidation in Leishmania mexicana
Description	Two cell-lines of Leishmania mexicana were investigated using a TMT-based approach; wild-type and an attenuated H-line. Half of each sample was fully reduced and all cysteine residues were labelled with the cysteine-reactive iodoTMT reagent, while the other half was reacted with N-ethylmaleimide to block all free cysteines before the remaining reversibly modified cysteines were reduced and labelled with iodoTMT. This allowed direct comparisons to be made between the level of modified cysteines and the total cysteine count.
HostingRepository	PRIDE
AnnounceDate	2018-11-23
AnnouncementXML	Submission_2018-11-23_08:49:20.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aruna Prakash
SpeciesList	scientific name: Leishmania mexicana MHOM/GT/2001/U1103; NCBI TaxID: 929439;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-30 01:22:38	ID requested
⏵ 1	2018-11-23 08:49:20	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

curator keyword: Biological
submitter keyword: Comparative proteomics, Leishmania mexicana, TMT, cysteine oxidation

curator keyword: Biological

submitter keyword: Comparative proteomics, Leishmania mexicana, TMT, cysteine oxidation

Contact List

Richard Burchmore
contact affiliation	Head of Proteomics at Glasgow Polyomics, College of Medical Veterinary and Life Sciences, University of Glasgow, UK
contact email	richard.burchmore@glasgow.ac.uk
lab head
Aruna Prakash
contact affiliation	University of Glasgow
contact email	a.s.prakash@dunelm.org.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010938
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD010938

PRIDE project URI

Repository Record List

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