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DataSet Summary

  • HostingRepository: PanoramaPublic
  • AnnounceDate: 2018-09-13
  • AnnouncementXML: Submission_2018-09-13_10:24:49.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Matt Foster
  • Title: Layers of regulation on cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae
  • Description: In the budding yeast Saccharomyces cerevisiae, transcription factors (TFs) regulate the periodic expression of many genes during the cell cycle, including gene products required for progression through cell-cycle events. Experimental evidence coupled with quantitative models suggest that a network of interconnected TFs is capable of regulating periodic genes over the cell cycle. Importantly, these dynamical models were built on transcriptomics data and assumed that TF protein levels and activity are directly correlated with mRNA abundance. To ask whether TF transcripts match protein expression levels as cells progress through the cell cycle, we applied a multiplexed targeted mass spectrometry approach (parallel reaction monitoring) on synchronized populations of cells. We found that protein expression of many TFs and cell-cycle regulators closely followed their respective mRNA transcript dynamics in cycling wild-type cells. Discordant mRNA/protein expression dynamics were also observed for a subset of cell-cycle TFs and for proteins targeted for degradation by E3 ubiquitin ligase complexes such as SCF (Skp1/Cul1/F-box) and APC/C (anaphase-promoting complex/cyclosome). We further profiled mutant cells lacking B-type cyclin/CDK activity (clb1-6), where oscillations in ubiquitin ligase activity, cyclin/CDKs, and cell-cycle progression are halted. We found that a number of proteins were no longer periodically degraded in clb1-6 mutants compared to wild type, highlighting the importance of post-transcriptional regulation. Finally, the TF complexes responsible for activating G1/S transcription (SBF and MBF) were more constitutively expressed at the protein level than their periodic mRNA expression levels in both wild-type and mutant cells. This comprehensive investigation of cell-cycle regulators reveals that multiple layers of regulation (transcription, protein stability, and proteasome targeting) affect protein expression dynamics during the cell cycle.
  • SpeciesList: scientific name: Saccharomyces cerevisiae; NCBI TaxID: 4932;
  • ModificationList: Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
  • Instrument: Q Exactive Plus

Dataset History

VersionDatetimeStatusChangeLog Entry
02018-08-29 15:31:26ID requested
12018-09-13 10:24:50announced

Publication List

  1. Kelliher CM, Foster MW, Motta FC, Deckard A, Soderblom EJ, Moseley MA, Haase SB, Layers of regulation of cell-cycle gene expression in the budding yeast Saccharomyces cerevisiae. Mol Biol Cell, 29(22):2644-2655(2018) [pubmed]

Keyword List

  1. submitter keyword: yeast, transcription factor, time-course

Contact List

    Steve Haase
    • contact affiliation: Duke University
    • contact email: steve.haase@duke.edu
    • lab head:
    Matt Foster
    • contact affiliation: Duke Proteomics and Metabolomics Shared Resource
    • contact email: mwfoster@duke.edu
    • dataset submitter:

Full Dataset Link List

  1. Panorama Public dataset URI

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