PXD010929 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | espL deletion mutant of Mycobacterium tuberculosis |
Description | The ESX-1, type VII, secretion system represents the major virulence determinant of Mycobacterium tuberculosis, one of the most successful intracellular pathogens. Here, by combining genetic and high-throughput approaches, we show that EspL, a protein of 115 amino acids, is essential for mediating ESX-1-dependent virulence and for stabilization of EspE, EspF and EspH protein levels. Indeed, an espL knock-out mutant was unable to replicate intracellularly, secrete ESX-1 substrates or stimulate innate cytokine production. Moreover, proteomic studies detected greatly reduced amounts of EspE, EspF and EspH in the espL mutant as compared to the wild type strain, suggesting a role for EspL as a chaperone. The latter conclusion was further supported by discovering that EspL interacts with EspD, which was previously demonstrated to stabilize the ESX-1 substrates and effector proteins, EspA and EspC. Moreover, loss of EspL also leads to downregulation in M. tuberculosis of WhiB6, a redox-sensitive transcriptional activator of ESX-1 genes. Overall, our data highlight the importance of a so-far overlooked, though conserved, component of the ESX-1 secretion system and begin to delineate the role played by EspE, EspF and EspH in virulence and host-pathogen interaction. |
HostingRepository | PRIDE |
AnnounceDate | 2019-12-13 |
AnnouncementXML | Submission_2019-12-13_07:34:50.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andrej Benjak |
SpeciesList | scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-29 07:16:46 | ID requested | |
1 | 2018-12-10 02:15:31 | announced | |
⏵ 2 | 2019-12-13 07:34:52 | announced | 2019-12-13: Updated publication reference for PubMed record(s): 30571761. |
Publication List
Sala C, Odermatt NT, Soler-Arnedo P, G, ΓΌ, len MF, von Schultz S, Benjak A, Cole ST, EspL is essential for virulence and stabilizes EspE, EspF and EspH levels in Mycobacterium tuberculosis. PLoS Pathog, 14(12):e1007491(2018) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: Mycobacterium, tuberculosis, espL, virulence |
Contact List
Stewart Thomas Cole |
contact affiliation | Global Health Institute, EPFL, Lausanne, Switzerland |
contact email | stewart.cole@epfl.ch |
lab head | |
Andrej Benjak |
contact affiliation | Global Health Institute |
contact email | andrej.benjak@epfl.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010929
- Label: PRIDE project
- Name: espL deletion mutant of Mycobacterium tuberculosis