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PXD010929

PXD010929 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleespL deletion mutant of Mycobacterium tuberculosis
DescriptionThe ESX-1, type VII, secretion system represents the major virulence determinant of Mycobacterium tuberculosis, one of the most successful intracellular pathogens. Here, by combining genetic and high-throughput approaches, we show that EspL, a protein of 115 amino acids, is essential for mediating ESX-1-dependent virulence and for stabilization of EspE, EspF and EspH protein levels. Indeed, an espL knock-out mutant was unable to replicate intracellularly, secrete ESX-1 substrates or stimulate innate cytokine production. Moreover, proteomic studies detected greatly reduced amounts of EspE, EspF and EspH in the espL mutant as compared to the wild type strain, suggesting a role for EspL as a chaperone. The latter conclusion was further supported by discovering that EspL interacts with EspD, which was previously demonstrated to stabilize the ESX-1 substrates and effector proteins, EspA and EspC. Moreover, loss of EspL also leads to downregulation in M. tuberculosis of WhiB6, a redox-sensitive transcriptional activator of ESX-1 genes. Overall, our data highlight the importance of a so-far overlooked, though conserved, component of the ESX-1 secretion system and begin to delineate the role played by EspE, EspF and EspH in virulence and host-pathogen interaction.
HostingRepositoryPRIDE
AnnounceDate2019-12-13
AnnouncementXMLSubmission_2019-12-13_07:34:50.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAndrej Benjak
SpeciesList scientific name: Mycobacterium tuberculosis H37Rv; NCBI TaxID: 83332;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-29 07:16:46ID requested
12018-12-10 02:15:31announced
22019-12-13 07:34:52announced2019-12-13: Updated publication reference for PubMed record(s): 30571761.
Publication List
Sala C, Odermatt NT, Soler-Arnedo P, G, ΓΌ, len MF, von Schultz S, Benjak A, Cole ST, EspL is essential for virulence and stabilizes EspE, EspF and EspH levels in Mycobacterium tuberculosis. PLoS Pathog, 14(12):e1007491(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: Mycobacterium, tuberculosis, espL, virulence
Contact List
Stewart Thomas Cole
contact affiliationGlobal Health Institute, EPFL, Lausanne, Switzerland
contact emailstewart.cole@epfl.ch
lab head
Andrej Benjak
contact affiliationGlobal Health Institute
contact emailandrej.benjak@epfl.ch
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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