PXD010927 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | microRNA-222 Attenuates Mitochondrial Dysfunction Induced by Transmissible Gastroenteritis Virus via Targeting Thrombospondin-1 and Inhibiting Cluster of Differentiation 47 |
Description | Transmissible gastroenteritis virus (TGEV) is a member of Coronaviridae family. Our previous research showed that TGEV infection could induce mitochondrial dysfunction and up-regulat miR-222 level. Therefore, we presumed that miR-222 might be implicated in regulating mitochondrial dysfunction induced by TGEV infection. To verify the hypothesis, the effect of miR-222 on mitochondrial dysfunction was detected and showed that miR-222 attenuated TGEV-induced mitochondrial dysfunction. To investigate the underlying molecular mechanism of miR-222 in TGEV-induced mitochondrial dysfunction, a quantitative proteomic analysis of PK-15 cells that were transfected with miR-222 mimics and infected with TGEV was performed. In total, 4151 proteins were quantified and 100 differentially expressed proteins were obtained (57 up-regulated, 43 down-regulated), among which thrombospondin-1 (THBS1) and cluster of differentiation 47 (CD47) were down-regulated. THBS1 was identified as the target of miR-222. Knockdown of THBS1 and CD47 increased mitochondrial Ca2+ level and decreased mitochondrial membrane potential (MMP) level. Together, our data establish a significant role of miR-222 in regulating mitochondrial dysfunction in response to TGEV infection. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:06:22.394.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD010927 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | lihuan Qin |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-29 04:36:43 | ID requested | |
1 | 2018-12-19 08:10:05 | announced | |
⏵ 2 | 2024-10-22 04:06:22 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: proteomics,microRNA-222, mitochondrial dysfunction, transmissible gastroenteritis virus, thrombospondin-1 (THBS1), cluster of differentiation 47 (CD47) |
Contact List
Dewen Tong |
contact affiliation | College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, P.R. China |
contact email | dwtong@nwsuaf.edu.cn |
lab head | |
lihuan Qin |
contact affiliation | PTM Biolabs Inc. |
contact email | lihuan_qin@ptm-biolab.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010927
- Label: PRIDE project
- Name: microRNA-222 Attenuates Mitochondrial Dysfunction Induced by Transmissible Gastroenteritis Virus via Targeting Thrombospondin-1 and Inhibiting Cluster of Differentiation 47