PXD010922

PXD010922 is an original dataset announced via ProteomeXchange.

Title	Zebrafish 24hpf whole embryo MZezh2mutant
Description	Polycomb group (PcG) proteins are transcriptional repressors important to maintain cell identity during embryonic development. Ezh2, the catalytic subunit of the Polycomb Repressive Complex 2, is responsible for placing the epigenetic repressive mark histone H3 lysine 27 trimethylation (H3K27me3). In contrast to results in mouse models, zebrafish embryos mutant for both maternal and zygotic ezh2 (MZezh2) can form a normal body plan at 1 day post fertilization (dpf) but die at 2 dpf, exhibiting pleiotropic phenotypes. To elucidate the specificity of PcG-mediated repression during early zebrafish development, we conducted in depth analysis of the transcriptome, epigenome, and proteome of the MZezh2 mutant embryos at 1 dpf. We found that, despite modifications in the epigenetic landscape, transcriptome and proteome analysis revealed only minor changes in gene and protein expression levels.
HostingRepository	PRIDE
AnnounceDate	2019-09-09
AnnouncementXML	Submission_2019-09-09_07:54:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leonie Kamminga
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-29 03:56:17	ID requested
⏵ 1	2019-09-09 07:54:38	announced

Rougeot J, Chrispijn ND, Aben M, Elurbe DM, Andralojc KM, Murphy PJ, Jansen PWTC, Vermeulen M, Cairns BR, Kamminga LM, Maintenance of spatial gene expression by Polycomb-mediated repression after formation of a vertebrate body plan. Development, 146(19):(2019) [pubmed]

curator keyword: Biological

submitter keyword: Zebrafish, 24hpf, embryo, ezh2

Leonie Kamminga
contact affiliation	Radboud University/Radboud UMC, Faculty of Science, Department of Molecular Biology, Radboud Institute for Molecular Life Sciences, Nijmegen, the Netherlands
contact email	l.kamminga@science.ru.nl
lab head
Leonie Kamminga
contact affiliation	Radboud University/ Radboud UMC, Faculty of Science, Department of Molecular Biology, Radboud Institute for Molecular Life Sciences, Nijmegen, the Netherlands
contact email	l.kamminga@science.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD010922

PRIDE project URI

• PRIDE
  1. PXD010922
     1. Label: PRIDE project
     2. Name: Zebrafish 24hpf whole embryo MZezh2mutant