PXD010921 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of CTPS1 Filament Interacting Proteins |
Description | CTP synthase (CTPS) catalyzes the formation of CTP in de novo pyrimidine biosynthesis pathway. Compartmentalization of CTPS into filamentous structure is evolutionarily conserved from E.coli, yeast, Drosophila, mice to humans. Recently, we have demonstrated that histidine-mediated protein methylation promotes the formation of CTPS filament under glutamine and/or serum deprivation, which reduces its enzymatic activity and protects it from degradation. However, it is still unclear how the filament assembly takes place. In the current study, we used APEX2-mediated in vivo proximity labeling to identify proteins involved in CTPS filament formation. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:01:20.924.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yu-Tsun Lin |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iTRAQ4plex-116 reporter+balance reagent acylated residue; methylthiolated residue; monohydroxylated residue; acetylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-29 03:24:02 | ID requested | |
1 | 2020-03-24 03:40:21 | announced | |
⏵ 2 | 2024-10-22 05:01:21 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1242/jcs.240200; |
Chakraborty A, Lin WC, Lin YT, Huang KJ, Wang PY, Chang IY, Wang HI, Ma KT, Wang CY, Huang XR, Lee YH, Chen BC, Hsieh YJ, Chien KY, Lin TY, Liu JL, Sung LY, Yu JS, Chang YS, Pai LM, SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network. J Cell Sci, 133(9):(2020) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: SNAP29, APEX2,CTPS, Cytokeratin |
Contact List
Jau-Song Yu |
contact affiliation | Molecular Medicine Research Center, Chang Gung University, Taoyuan 33302, Taiwan |
contact email | yusong@mail.cgu.edu.tw |
lab head | |
Yu-Tsun Lin |
contact affiliation | Chang Gung University |
contact email | doreenlin1230@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010921
- Label: PRIDE project
- Name: Identification of CTPS1 Filament Interacting Proteins