PXD010918
PXD010918 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Plasma CFH (Zhang et al. 2017) |
| Description | Age-related macular degeneration (AMD) is a leading cause of visual loss among older adults. Two variants in the complement factor H (CFH) gene, Y402H and I62V, are strongly associated with risk of AMD. CFH is encoded in the Regulator of Complement Activation gene cluster in chromosome 1q32, which includes complement factor-related (CFHR) proteins, CFHR1 to CFHR5, with high amino acid sequence homology to CFH. Our goal was to build a selected reaction monitoring (SRM) assay to measure plasma concentrations of CFH variants Y402, H402, I62, and V62, and CFHR1-5. The final assay consisted of 36 peptides and 108 interference-free SRM transition ion pairs. Most peptides showed good linearity over 0.3-200 fmol/μL concentration range. Plasma concentrations of CFH variants and CFHR1-5 were measured using the SRM assay in 344 adults. Plasma CFH concentrations (mean, SE in μg/mL) by genotype were: YY402, II62 (170.1, 31.4), YY402, VV62 (188.8, 38.5), HH402, VV62 (144.0, 37.0), HY402, VV62 (164.2, 42.3), YY402, IV62 (194.8, 36.8), HY402, IV62 (181.3, 44.7). Mean (SE) plasma concentrations of CFHR1-5 were 1.63 (0.04), 3.64 (1.20), 0.020 (0.001), 2.42 (0.18), and 5.49 (1.55) μg/mL, respectively. This SRM assay should facilitate the study of the role of systemic complement and risk of AMD. |
| HostingRepository | PeptideAtlas |
| AnnounceDate | 2018-08-28 |
| AnnouncementXML | Submission_2018-08-28_15:14:38.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Pingbo Zhang |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | 6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine; S-carboxamidomethyl-L-cysteine |
| Instrument | QTRAP 5500 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2018-08-28 15:13:38 | ID requested | |
| ⏵ 1 | 2018-08-28 15:14:39 | announced |
Publication List
| Zhang P, Zhu M, Geng-Spyropoulos M, Shardell M, Gonzalez-Freire M, Gudnason V, Eiriksdottir G, Schaumberg D, Van Eyk JE, Ferrucci L, Semba RD, A novel, multiplexed targeted mass spectrometry assay for quantification of complement factor H (CFH) variants and CFH-related proteins 1-5 in human plasma. Proteomics, 17(6):(2017) [pubmed] |
Keyword List
| curator keyword: selected reaction monitoring, SRM, targeted |
Contact List
| Pingbo Zhang | |
|---|---|
| contact affiliation | Johns Hopkins University School of Medicine |
| contact email | pzhang7@jhmi.edu |
| dataset submitter | |
| Richard D. Semba | |
| contact affiliation | Wilmer Eye Institute, Johns Hopkins University School of Medicine |
| contact email | rdsemba@jhmi.edu |
| lab head | |
Full Dataset Link List
| PeptideAtlas dataset URI |
| PASSEL experiment URI |
| PASSEL transition group browser URI |
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