PXD010917

PXD010917 is an original dataset announced via ProteomeXchange.

Title	TUPA0002 (Williams et al. 2017)
Description	Purpose: Development of delayed graft function (DGF) following kidney transplant is associated with poor outcomes. An ability to distinguish patients, promptly after surgery, with DGF versus immediate graft function (IGF) based on the differential expression of urine protein biomarkers may facilitate treatment and the research needed to improve prognosis. The purpose of this study was to use a Targeted Urine Proteome Assay (TUPA), which was described in Cantley et al, 2016 (PMID: 26220717, PMCID in progress), to identify protein biomarkers of delayed recovery from kidney transplant. Experimental Design: Potential biomarkers were identified by using the TUPA Multiple Reaction Monitoring (MRM) assay to interrogate the relative IGF/DGF levels of expression of up to 167 proteins in urine from 21 DGF, 15 SGF (slow graft function), and 16 IGF patients. An iterative Random Forest analysis approach evaluated the relative importance of each biomarker, which was then used to identify an optimum biomarker panel that provides the maximum sensitivity and specificity with the least number of biomarkers. Conclusions and clinical relevance: With a sensitivity of 82.6%, specificity of 77.4% and AUC of 0.891, the Â?Top 4Â? proteins have significant ability to identify DGF. This panel represents an important step towards identifying DGF at an early stage so that more effective treatments can be developed to improve long term graft outcomes.
HostingRepository	PeptideAtlas
AnnounceDate	2018-08-28
AnnouncementXML	Submission_2018-08-28_15:14:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kenneth Williams
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine
Instrument	QTRAP 5500

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-28 15:12:26	ID requested
⏵ 1	2018-08-28 15:14:34	announced

Williams KR, Colangelo CM, Hou L, Chung L, Belcher JM, Abbott T, Hall IE, Zhao H, Cantley LG, Parikh CR, Use of a Targeted Urine Proteome Assay (TUPA) to identify protein biomarkers of delayed recovery after kidney transplant. Proteomics Clin Appl, 11(7-8):(2017) [pubmed]

curator keyword: selected reaction monitoring, SRM, targeted

Kenneth Williams
contact affiliation	Yale University School of Medicine
contact email	kenneth.williams@yale.edu
dataset submitter
Chirag R. Parikh
contact affiliation	Yale University School of Medicine
contact email	chirag.parikh@yale.edu
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI