PXD010911

PXD010911 is an original dataset announced via ProteomeXchange.

Title	Human Serum Immunoglobulin N-Glycosylation using nLC-MS/MS
Description	Here we employ a nLC-MS/MS method to separate small amounts of purified immunoglobulins to characterize the N-glycan reportoire and site occupancy of bulk serum antibodies. Using this method, we have established, for the first time within individual donors, the N-linked glycan repotoire for bulk IgG1, IgG4, IgA1, IgA2 and IgM in healthy individuals. This is crucial for developing a platform to define disease-specific N-glycan signatures for different isotypes to help tune antibodes to induce protection.
HostingRepository	PRIDE
AnnounceDate	2019-01-25
AnnouncementXML	Submission_2019-01-25_05:10:46.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010911
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kevin Chandler
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Delta:H(1)O(-1)18O(1); Oxidation; Carbamidomethyl; Oxidation
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-28 08:19:09	ID requested
⏵ 1	2019-01-25 05:10:47	announced

Chandler KB, Mehta N, Leon DR, Suscovich TJ, Alter G, Costello CE, -Glycosylation Profiles. Mol Cell Proteomics, 18(4):686-703(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Serum, IgG1, IgG4, IgA1, IgA2, IgM, Immunoglobulins, N-glycosylation, glycopeptides

Catherine E Costello
contact affiliation	Prof. Catherine E. Costello Center for Biomedical Mass Spectrometry Boston University School of Medicine 670 Albany St, Rm 511 Boston, MA 02118-2646 USA Ph.: (+1) 617-638-6490 Fax: (+1) 617-638-6761 Email: cecmsms@bu.edu
contact email	cecmsms@bu.edu
lab head
Kevin Chandler
contact affiliation	Boston University
contact email	kbc36@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010911

PRIDE project URI

• PRIDE
  1. PXD010911
     1. Label: PRIDE project
     2. Name: Human Serum Immunoglobulin N-Glycosylation using nLC-MS/MS