PXD010910

PXD010910 is an original dataset announced via ProteomeXchange.

Title	Plasma functionalization of PCL nanofibers changes protein interactions with cells resulting in increased cell viability
Description	The surface properties of electrospun scaffolds can greatly influence protein adsorption and thus strongly dictate cell-material interactions. In this study, we aim to investigate possible correlations between the surface properties of argon, nitrogen and ammonia/helium plasma-functionalized polycaprolactone (PCL) nanofibers and their cellular interactions by examining the protein corona patterns of the plasma-treated nanofibers as well as the cell membrane proteins involved in cell proliferation. As a result of the performed plasma treatments, PCL nanofiber morphology was preserved while wettability was improved profoundly after all treatments because of the incorporation of polar surface groups. Depending on the discharge gas, different types of groups are incorporated which influenced the resultant cell-material interactions. Argon plasma-functionalized PCL nanofibers, only enriched by oxygen-containing functional groups, were found to show the best cell-material interactions, followed by N2 and He/NH3 plasma-treated samples. SDS-PAGE and LC-MS clearly indicated an increased protein retention compared to non-treated PCL NFs. The most differential proteins surface a distinct molecular machinery mediating the increased proliferation and viability on plasma treated NF. Finally, differential protein analysis between the different plasma’s gives a first hint into the molecular underpinning of the superior performance of Argon NF.
HostingRepository	PRIDE
AnnounceDate	2018-12-04
AnnouncementXML	Submission_2019-06-17_07:23:57.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010910
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Maarten Dhaenens
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-28 08:15:48	ID requested
1	2018-12-04 04:06:35	announced
2	2018-12-04 04:07:11	announced	Updated project metadata.
⏵ 3	2019-06-17 07:23:58	announced	Updated project metadata.

Asadian M, Dhaenens M, Onyshchenko I, De Waele S, Declercq H, Cools P, Devreese B, Deforce D, Morent R, De Geyter N, Plasma Functionalization of Polycaprolactone Nanofibers Changes Protein Interactions with Cells, Resulting in Increased Cell Viability. ACS Appl Mater Interfaces, 10(49):41962-41977(2018) [pubmed]

curator keyword: Biological

submitter keyword: Human, HFF, SynaptG2Si, Functionalized plasma, nanofibres

Dieter Deforce
contact affiliation	Pharmaceutical Biotechnologu, Faculty of Pharmaceutical Biotechnology, Ghent University, Belgium
contact email	Dieter.deforce@ugent.be
lab head
Maarten Dhaenens
contact affiliation	Faculity of Pharmaceutical Biotechnology
contact email	maarten.dhaenens@ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010910
     1. Label: PRIDE project
     2. Name: Plasma functionalization of PCL nanofibers changes protein interactions with cells resulting in increased cell viability