PXD010900

PXD010900 is an original dataset announced via ProteomeXchange.

Title	To invesigate the role of autophagy in Arabidopsis root cellular protein turnover and proteostasis
Description	Protein turnover analysis using partial 15N labelling and protein abundance analysis using 15N spike-in were coupled to investiage the role of autophagy in protein degradation and proteostasis. Our findings show that dysfuncional autophagy due to loss-of-function autophagy components ATG5 and 11 lead to accumulation of protein targets with/without starvation stress. Further protein turnover analysis uncover a subgroup of proteins with retarded degradation. New selective autophagy protein targets involving glycolytic proteins were uncovered by this strategy. Furthermore, we found plasma membrane and cytoskeleton proteins show a general reduction, supporting the cross-interaction between autopahgy and vesicle trafficking pathway. Metabolomics changes in autophagy mutants due to a changed proteostasis was further investigated.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_11:38:36.554.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Li
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	6520A Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-28 05:59:47	ID requested
⏵ 1	2024-10-08 11:38:37	announced

10.1093/plcell/koac185;

Li L, Lee CP, Ding X, Qin Y, Wijerathna-Yapa A, Broda M, Otegui MS, Millar AH, Defects in autophagy lead to selective in vivo changes in turnover of cytosolic and organelle proteins in Arabidopsis. Plant Cell, 34(10):3936-3960(2022) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, 15N labelling, protein turnover, autophagy

Harvey Millar
contact affiliation	ARC Centre of Excellence in Plant Energy Biology, M316, 35 Stirling Hwy, 6009, WA, Australia Faculty of Science, The University of Western Australia
contact email	harvey.millar@uwa.edu.au
lab head
Lei Li
contact affiliation	College of Life Sciences, Nankai University, Tianjin 300071
contact email	lei.li@nankai.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD010900

PRIDE project URI

• PRIDE
  1. PXD010900
     1. Label: PRIDE project
     2. Name: To invesigate the role of autophagy in Arabidopsis root cellular protein turnover and proteostasis