PXD010887

PXD010887 is an original dataset announced via ProteomeXchange.

Title	micro-proteomics experiment for single meiotic-phase barley anthers
Description	We describe here a new micro-proteomics workflow for the analysis of individual barley anthers. The workflow detects more than 4000 proteins from small amounts of material representing single or paired anther samples, covering a dynamic range of protein expression levels across five orders of magnitude. We consistently identified ~2800 proteins in a single 0.6mm anther and ~4000 proteins in a paired-anther sample. This demonstrates an important technological advance in plant proteomics, considering the limited amount of starting material. We employed our micro-proteomics workflow to investigate the proteome of the developing barley anther containing male gametes in the early stages of meiosis. We successfully identified several low abundant meiosis-related proteins, proving that our approach is highly sensitive and consequently powerful. To date, no work has been published previously on precisely staged individual plant anthers.
HostingRepository	PRIDE
AnnounceDate	2019-05-04
AnnouncementXML	Submission_2019-05-04_02:17:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Runxuan Zhang
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-28 02:13:36	ID requested
⏵ 1	2019-05-04 02:17:09	announced

Lewandowska D, Zhang R, Colas I, Uzrek N, Waugh R, Application of a Sensitive and Reproducible Label-Free Proteomic Approach to Explore the Proteome of Individual Meiotic-Phase Barley Anthers. Front Plant Sci, 10():393(2019) [pubmed]

curator keyword: Biological

submitter keyword: micro-proteomics, barley, anther

Robbie Waugh
contact affiliation	The James Hutton Institute
contact email	robbie.waugh@hutton.ac.uk
lab head
Runxuan Zhang
contact affiliation	The James Hutton Institute
contact email	runxuan.zhang@hutton.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD010887

PRIDE project URI

• PRIDE
  1. PXD010887
     1. Label: PRIDE project
     2. Name: micro-proteomics experiment for single meiotic-phase barley anthers