PXD010886

PXD010886 is an original dataset announced via ProteomeXchange.

Title	Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis
Description	During plant vascular development, xylem tracheary elements (TEs) form water conducting, empty pipes through genetically regulated cell death. Cell death is prevented from spreading to non-TEs by unidentified intercellular mechanisms, downstream of METACASPASE9 (MC9)-mediated regulation of autophagy in TEs. Here, we identified differentially abundant extracellular peptides in vascular11 differentiating wild type and MC9-downregulated Arabidopsis cell suspensions. The peptide Kratos rescued the abnormally high ectopic non-TE death resulting from either MC9 knockout or TE-specific overexpression of the ATG5 autophagy protein during experimentally induced vascular differentiation in Arabidopsis cotyledons. Kratos also reduced cell death following mechanical damage and extracellular ROS production in Arabidopsis leaves. Stress-induced but not vascular non-TE cell death was enhanced by another identified peptide, Bia. Bia is therefore reminiscent of several known plant cell death-inducing peptides acting as damage-associated molecular patterns. In contrast, Kratos plays a novel extracellular cell survival role in the context of development and during stress response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:12:55.926.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrick Willems
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-28 02:10:59	ID requested
1	2019-01-31 06:33:41	announced
2	2019-05-31 02:25:17	announced	Updated publication reference for PubMed record(s): 30753577.
⏵ 3	2024-10-22 04:12:56	announced	2024-10-22: Updated project metadata.

10.1093/jxb/erz021;

Escamez S, Stael S, Vainonen JP, Willems P, Jin H, Kimura S, Van Breusegem F, Gevaert K, Wrzaczek M, Tuominen H, Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis. J Exp Bot, 70(7):2199-2210(2019) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, vascular development, tracheary element,Peptidomics, extracellular, cell death, METACASPASE9

Kris Gevaert
contact affiliation	VIB-UGent Center for Medical Biotechnology
contact email	kris.gevaert@ugent.vib.be
lab head
Patrick Willems
contact affiliation	PSB-VIB
contact email	pawil@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010886

PRIDE project URI

• PRIDE
  1. PXD010886
     1. Label: PRIDE project
     2. Name: Extracellular peptide Kratos restricts cell death during vascular development and stress in Arabidopsis