PXD010881

PXD010881 is an original dataset announced via ProteomeXchange.

Title	A proteomic and functional analysis of the metabolic adaptive response to iron during the filamentation process of the pathogen Candida albicans.
Description	Candia albicans is an opportunist pathogen responsible for a large spectrum of infections, from superficial mycosis to systemic diseases called candidiasis. Its ability to grow in various morphological forms, such as yeast and filamentous hyphae, contributes to its survival in the diverse microenvironments it encounters in the host, differing in key micronutrients and iron contents. In C. albicans, iron uptake has been associated with virulence and the pathogen has developed elaborated strategies to acquire iron from the host. In this study, we performed a global proteomic analysis of the changes of protein abundances in response to iron, and correlated these data with morphological and functional studies of the main metabolic pathways of the pathogen, both in the yeast and filamentous forms. Our results show that C. albicans yeast cells have an extraordinary capability to adapt to both iron excess and iron deficiency. Moreover, changes of iron content in the growth medium are associated with a metabolic remodeling affecting intracellular glutathione levels and reduced thiol groups, but also enzymes of the TCA cycle, the pentose phosphate pathway or the mitochondrial respiratory chain. Our data indicate that iron-deficiency stimulates the bioenergetics pathways, whereas iron excess corresponds to more biosynthetic needs, especially in the filamentous forms. Altogether, our results emphasize the influence of the environment of C. albicans cells on the filamentation process, and show evidence for the differences between the yeast and filamentous forms, which seem to adapt more easily in response to a large set of iron conditions.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:04:53.157.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Camille GARCIA
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	S-nitrosyl-L-cysteine; phosphorylated residue; monohydroxylated residue; acetylated residue; cysteine 4-hydroxynonenal adduct; L-cysteine glutathione disulfide
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-28 01:41:49	ID requested
1	2020-05-26 12:21:40	announced
⏵ 2	2024-10-22 05:05:01	announced	2024-10-22: Updated project metadata.

Duval C, Macabiou C, Garcia C, Lesuisse E, Camadro JM, Auch, è, re F, The adaptive response to iron involves changes in energetic strategies in the pathogen Candida albicans. Microbiologyopen, 9(2):e970(2020) [pubmed]

10.1002/mbo3.970;

curator keyword: Biological, Biomedical

submitter keyword: Albicans-Iron-Filamentation

Jean-Michel Camadro
contact affiliation	Laboratoire Mitochondries, Métaux et Stress Oxydant, Institut Jacques Monod, UMR 7592, Université Paris-Diderot/CNRS (USPC), 15 rue Hélène Brion, 75013 Paris, France
contact email	jean-michel.camadro@ijm.fr
lab head
Camille GARCIA
contact affiliation	Institut Jacques Monod
contact email	camille.garcia@ijm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD010881

PRIDE project URI

• PRIDE
  1. PXD010881
     1. Label: PRIDE project
     2. Name: A proteomic and functional analysis of the metabolic adaptive response to iron during the filamentation process of the pathogen Candida albicans.