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PXD010878 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDT2216 is a specific BCL-XL PROTAC
DescriptionTo validate the specificity of DT2216, we used the stable isotope labeling with amino acids in cell culture (SILAC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics to analyze the changes in proteins in WI-38 normal human diploid fibroblasts after DT2216 and DT2216NC treatment. The results show that DT2216, but not DT2216NC, reduced the levels of BCL-XL, but none of other proteins were significantly affected by either agent, demonstrating that DT2216 is a specific BCL-XL PROTAC.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDongwen Lv
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-28 01:27:57ID requested
12019-10-08 07:04:21announced
22019-12-09 23:36:26announced2019-12-10: Updated publication reference for PubMed record(s): 31792461.
Publication List
Khan S, Zhang X, Lv D, Zhang Q, He Y, Zhang P, Liu X, Thummuri D, Yuan Y, Wiegand JS, Pei J, Zhang W, Sharma A, McCurdy CR, Kuruvilla VM, Baran N, Ferrando AA, Kim YM, Rogojina A, Houghton PJ, Huang G, Hromas R, Konopleva M, Zheng G, Zhou D, PROTAC degrader achieves safe and potent antitumor activity. Nat Med, 25(12):1938-1947(2019) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: DT2216 Bcl-xl PROTAC SILAC LC-MS/MS
Contact List
Daohong Zhou
contact affiliationDepartment of Pharmacodynamics, University of Florida
contact emailzhoudaohong@cop.ufl.edu
lab head
Dongwen Lv
contact affiliationUniversity of Florida
contact emaillyu.dongwen@cop.ufl.edu
dataset submitter
Full Dataset Link List
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PRIDE project URI
Repository Record List
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