PXD010878

PXD010878 is an original dataset announced via ProteomeXchange.

Title	DT2216 is a specific BCL-XL PROTAC
Description	To validate the specificity of DT2216, we used the stable isotope labeling with amino acids in cell culture (SILAC) and liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics to analyze the changes in proteins in WI-38 normal human diploid fibroblasts after DT2216 and DT2216NC treatment. The results show that DT2216, but not DT2216NC, reduced the levels of BCL-XL, but none of other proteins were significantly affected by either agent, demonstrating that DT2216 is a specific BCL-XL PROTAC.
HostingRepository	PRIDE
AnnounceDate	2019-10-08
AnnouncementXML	Submission_2019-12-09_23:36:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongwen Lv
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-28 01:27:57	ID requested
1	2019-10-08 07:04:21	announced
⏵ 2	2019-12-09 23:36:26	announced	2019-12-10: Updated publication reference for PubMed record(s): 31792461.

Khan S, Zhang X, Lv D, Zhang Q, He Y, Zhang P, Liu X, Thummuri D, Yuan Y, Wiegand JS, Pei J, Zhang W, Sharma A, McCurdy CR, Kuruvilla VM, Baran N, Ferrando AA, Kim YM, Rogojina A, Houghton PJ, Huang G, Hromas R, Konopleva M, Zheng G, Zhou D, PROTAC degrader achieves safe and potent antitumor activity. Nat Med, 25(12):1938-1947(2019) [pubmed]

curator keyword: Biological

submitter keyword: DT2216 Bcl-xl PROTAC SILAC LC-MS/MS

Daohong Zhou
contact affiliation	Department of Pharmacodynamics, University of Florida
contact email	zhoudaohong@cop.ufl.edu
lab head
Dongwen Lv
contact affiliation	University of Florida
contact email	lyu.dongwen@cop.ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD010878

PRIDE project URI

• PRIDE
  1. PXD010878
     1. Label: PRIDE project
     2. Name: DT2216 is a specific BCL-XL PROTAC