PXD010877

PXD010877 is an original dataset announced via ProteomeXchange.

Title	Tungsten stress response of soy bean (glycine max), LC-MSMS
Description	The transition metal tungsten (W) finds increasing application in military, aviation and household appliance industry, opening new paths for the heavy metal into the environment. Since tungsten shares certain chemical properties with the essential plant micro nutrient molybdenum, it is proposed to inhibit enzymatic activity of molybdoenzymes by replacing the Mo-ion bound to the co-factor. However, recent studies suggest the inhibition of enzymatic activity might not be the only effect W has on plants and that, much like other heavy metals, tungsten exerts toxicity on its own. Still, our understanding of the mechanisms behind the apparent phytotoxicity remains limited.This study investigates the effects of W on growth, nutrient levels (ionome, ICP-MS), starch levels and nitrogen nutrition (IRMS, enzyme activity assays) as well as root and nodule proteome (LC-MS/MS) of Glycine max cv. Primus. Plants were inoculated with Bradyrhizobium japonicum and grown in a semi hydroponic set up using three different tungsten concentrations (zero, 0.1 mM and 0.5 mM). To identify possible benefits of a functional bacterial symbiosis on W induced stress response, two different environmental growth conditions, one with suppressed N-fixation, supplied with Nitrate (10 mM KNO3) and one solely relying on symbiotic N-fixation (zero KNO3), were applied. Glycine max was able to take up considerable amounts of W (703 ± 136 mg kg-1). However, high W resulted in a strong reduction of shoot biomass of both N regimes and of root biomass (N fed only). Irrespective of N regime, NR activity and total N decreased with increasing W concentrations. Similarly, nitrogenase precursor levels as well as N2-fixation, nodule fixation activity (mg N g-1 nodule dry weight) and nodulation were reduced, indicating that nitrogenase synthesis and activity were negatively affected by W stress. However, along a N fix nodule specific induction of the secondary and phytohormone metabolism, it appears that N2 fixation of remaining nodules was not as severely affected by increasing levels of tungsten than nitrate reduction. Besides N metabolism, plants exhibited an imbalance in nutrient and a failure of carbon metabolic pathways accompanied by an accumulation of starch at high tungsten concentrations, independent of N-regime. Proteomic data demonstrated that the response to high W concentrations is independent of nodule functionality and dominated by several peroxidases and other general stress related proteins. Based on an evaluation of several W responsive proteotypic peptides, we identified a set of protein markers of W stress and possible targets for improved stress tolerance.
HostingRepository	PRIDE
AnnounceDate	2019-04-09
AnnouncementXML	Submission_2019-05-14_07:23:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Preiner
SpeciesList	scientific name: Bradyrhizobium japonicum; NCBI TaxID: 375; scientific name: Glycine max (Soybean) (Glycine hispida); NCBI TaxID: 3847;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-28 00:33:31	ID requested
1	2019-04-09 02:36:59	announced
⏵ 2	2019-05-14 07:23:07	announced	Updated publication reference for PubMed record(s): 31001297.

Preiner J, Wienkoop S, Weckwerth W, Oburger E, Depending on Nitrogen Regime. Front Plant Sci, 10():367(2019) [pubmed]

curator keyword: Biological

submitter keyword: Soy bean, glycine max, heavy metal, tungsten, legume

Stefanie Wienkoop
contact affiliation	Department of Ecogenomics and Systems Biology, Plant-Systems Interaction Group, University of Vienna
contact email	stefanie.wienkoop@univie.ac.at
lab head
Julian Preiner
contact affiliation	University of Vienna
contact email	julian.preiner@univie.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/04/PXD010877

PRIDE project URI

• PRIDE
  1. PXD010877
     1. Label: PRIDE project
     2. Name: Tungsten stress response of soy bean (glycine max), LC-MSMS