PXD010865 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Peptide mapping of purified huntingtin protein samples from different eukaryotic expression systems |
Description | Huntingtin expression, purification and biophysical characterisation: purified huntingtin protein samples of different polyQ lengths derived from insect cell and mammalian cell production were digested and subject to peptide mass spectrometry. |
HostingRepository | PRIDE |
AnnounceDate | 2019-03-12 |
AnnouncementXML | Submission_2019-03-12_11:12:48.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Suzanne Ackloo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Spodoptera frugiperda; NCBI TaxID: 7108; |
ModificationList | methylated residue |
Instrument | 6545 Q-TOF LC/MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-24 08:15:45 | ID requested | |
⏵ 1 | 2019-03-12 11:12:49 | announced | |
Publication List
Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH, Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems. J Biol Chem, 294(17):6986-7001(2019) [pubmed] |
Keyword List
curator keyword: Technical, Biological |
submitter keyword: Huntingtin, HTT, Huntington’s disease, HD, polyglutamine, polyQ, post-translational modification, eukaryotic expression system, pBacMam |
Contact List
Cheryl H Arrowsmith |
contact affiliation | Structural Genomics Consortium, University of Toronto, Toronto ON M5G 1L7, Canada. Tel.: 416-946-0881 E-mail: cheryl.arrowsmith@uhnresearch.ca |
contact email | cheryl.arrowsmith@uhnresearch.ca |
lab head | |
Suzanne Ackloo |
contact affiliation | University of Toronto |
contact email | suzanne.ackloo@utoronto.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD010865 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010865
- Label: PRIDE project
- Name: Peptide mapping of purified huntingtin protein samples from different eukaryotic expression systems