PXD010865

PXD010865 is an original dataset announced via ProteomeXchange.

Title	Peptide mapping of purified huntingtin protein samples from different eukaryotic expression systems
Description	Huntingtin expression, purification and biophysical characterisation: purified huntingtin protein samples of different polyQ lengths derived from insect cell and mammalian cell production were digested and subject to peptide mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2019-03-12
AnnouncementXML	Submission_2019-03-12_11:12:48.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suzanne Ackloo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Spodoptera frugiperda; NCBI TaxID: 7108;
ModificationList	methylated residue
Instrument	6545 Q-TOF LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-24 08:15:45	ID requested
⏵ 1	2019-03-12 11:12:49	announced

Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith CH, Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems. J Biol Chem, 294(17):6986-7001(2019) [pubmed]

curator keyword: Technical, Biological

submitter keyword: Huntingtin, HTT, Huntington’s disease, HD, polyglutamine, polyQ, post-translational modification, eukaryotic expression system, pBacMam

Cheryl H Arrowsmith
contact affiliation	Structural Genomics Consortium, University of Toronto, Toronto ON M5G 1L7, Canada. Tel.: 416-946-0881 E-mail: cheryl.arrowsmith@uhnresearch.ca
contact email	cheryl.arrowsmith@uhnresearch.ca
lab head
Suzanne Ackloo
contact affiliation	University of Toronto
contact email	suzanne.ackloo@utoronto.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD010865

PRIDE project URI

• PRIDE
  1. PXD010865
     1. Label: PRIDE project
     2. Name: Peptide mapping of purified huntingtin protein samples from different eukaryotic expression systems