PXD010850

PXD010850 is an original dataset announced via ProteomeXchange.

Title	Translocase of the Outer Membrane (TOM complex) of hydrogenosomes in Trichomonas vaginalis
Description	Mitochondria originated from α-proteobacterial endosymbionts, and the endosymbiont-to-organelle transition is tightly linked to the establishment of protein import pathways. The initial import of most proteins is mediated by the translocase of the outer membrane (TOM). Although TOM is common to all forms of mitochondria, recent studies have shown diversity of TOM subunits between main eukaryotic lineages. However, experimental knowledge is currently limited to a few model organisms. Here, we analysed the TvTOM complex in hydrogenosomes, an anaerobic form of mitochondria found in the excavate Trichomonas vaginalis. Using the pore-forming TvTom40-2 as bait, we immunoprecipitated the high molecular weight TvTOM complex both under crosslinking and native conditions and performed label-free quantitative mass spectrometry (LFQ-MS) to identify the components of the TvTOM complex. Further, we used two proteins that were enriched, Homp36 and Sam50 as baits for reciprocal experiments.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:13:38.868.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Abhijith Makki
SpeciesList	scientific name: Trichomonas vaginalis G3; NCBI TaxID: 412133;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-23 06:54:36	ID requested
1	2019-01-07 06:39:48	announced
⏵ 2	2024-10-22 04:13:39	announced	2024-10-22: Updated project metadata.

Makki A, Rada P, Ž, á, rsk, ý V, Kere, ï, che S, Kov, á, č, ik L, Novotn, ý M, Jores T, Rapaport D, Tachezy J, Triplet-pore structure of a highly divergent TOM complex of hydrogenosomes in Trichomonas vaginalis. PLoS Biol, 17(1):e3000098(2019) [pubmed]

10.1371/journal.pbio.3000098;

ProteomeXchange project tag: Mitochondria (HPP)

curator keyword: Biological

submitter keyword: Mitochondria,TOM complex, Trichomonas vaginalis, Protein import/translocation, Hydrogenosomes

Jan Tachezy
contact affiliation	Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Průmyslová 595, 25242 Vestec, Czech Republic
contact email	jan.tachezy@natur.cuni.cz
lab head
Abhijith Makki
contact affiliation	BIOCEV, Charles University
contact email	abhijith.makki@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010850

PRIDE project URI

• PRIDE
  1. PXD010850
     1. Label: PRIDE project
     2. Name: Translocase of the Outer Membrane (TOM complex) of hydrogenosomes in Trichomonas vaginalis