PXD010849

PXD010849 is an original dataset announced via ProteomeXchange.

Title	Reactive oxygen species (ROS) production activates unconventional secretion of antioxidant proteins
Description	Signal-sequence-lacking superoxide dismutase 1 (SOD1) is secreted upon nutrient starvation, but the physiological relevance of this secretion is unclear. We now report that secreted SOD1 is functionally active. In addition, a secretome analysis has revealed number of other secreted cytoplasmic antioxidant enzymes, including both thioredoxins, (Trx1 and Trx2), and the peroxiredoxin Ahp1. Our data reveal that starvation triggers increased mitochondrial activity, leading to increased production of reactive oxygen species (ROS). Inhibiting mitochondrial activity or ROS, inhibited secretion of the antioxidants. We suggest elevated ROS that evade cytoplasmic antioxidants, can permeate across the plasma membrane to the extracellular space. Cells secrete antioxidants to prevent ROS-mediated damage to the extracellular space. These findings thus provide an understanding of why cells secrete signal sequence lacking proteins like SOD1 and the larger family of antioxidants.
HostingRepository	PRIDE
AnnounceDate	2019-05-14
AnnouncementXML	Submission_2019-05-14_05:36:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eduard Sabidó
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-23 06:53:02	ID requested
⏵ 1	2019-05-14 05:36:53	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: ROS, antioxidant, unconventional secretion

Amy Curwin
contact affiliation	Center for Genomic Regulation, 08003 Barcelona, Spain
contact email	amy.curwin@crg.eu
lab head
Eduard Sabidó
contact affiliation	Centre de Regulació Genòmica
contact email	eduard.sabido@crg.cat
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010849
     1. Label: PRIDE project
     2. Name: Reactive oxygen species (ROS) production activates unconventional secretion of antioxidant proteins