PXD010844 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomic analysis provides novel insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.) |
Description | Cucumber (Cucumis sativus L.) is an economically important vegetable crop distributed in over 80 countries. Downy mildew (DM) caused by the obligate oomycete Pseudoperonospora cubensis is especially destructive in cucumber production. So far, few studies on the changes in proteomes during the P. cubensis infection have been performed. Using a newly developed TMT-LC-MS/MS analysis, the proteomes of DM-resistant variety ‘ZJ’ and DM-susceptible variety ‘SDG’ under the P. cubensis infection were investigated. In total, 6400 proteins were identified, 5629 of which were quantified. The differential accumulated proteins (DAPs) exhibited various biological functions and diverse subcellular localizations. KEGG enrichment analysis showed that various metabolic pathways were significantly altered under the P. cubensis infection, such as terpenoid backbone biosynthesis, and selenocompound metabolism in ZJ, and starch and sucrose metabolism in SDG. Most of the enzymes associated with terpenoid backbone synthesis were significantly accumulated in ZJ rather than in SDG, suggesting that pathogen-induced terpenoids accumulation might play an important role in the resistance against P. cubensis infection. Furthermore, a number of pathogenesis-related proteins and heat shock proteins were identified as DAPs, suggesting that DM resistance was controlled by a complex network. Our data allowed us to identify and screen more potential proteins related to the DM resistance. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:13:52.965.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | xiaonan fu |
SpeciesList | scientific name: Cucumis sativus; NCBI TaxID: 3659; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-23 06:51:28 | ID requested | |
1 | 2019-11-12 13:45:45 | announced | |
⏵ 2 | 2024-10-22 04:13:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
Zhang P, Zhu Y, Luo X, Zhou S, Comparative proteomic analysis provides insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.). Sci Rep, 9(1):9433(2019) [pubmed] |
10.1038/s41598-019-45111-4; |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: downy mildew,cucumber, terpenoid backbone biosynthesis, pathogen resistance, differential accumulated protein, Pseudoperonospora cubensis |
Contact List
Shengjun Zhou |
contact affiliation | Institute of Vegetable, Zhejiang Academy of Agriculture Sciences, Hangzhou, China |
contact email | yinxiang0586@sohu.com |
lab head | |
xiaonan fu |
contact affiliation | zhejiang scientific technology universitiy |
contact email | 2016460649@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD010844 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010844
- Label: PRIDE project
- Name: Comparative proteomic analysis provides novel insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.)