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PXD010844

PXD010844 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomic analysis provides novel insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.)
DescriptionCucumber (Cucumis sativus L.) is an economically important vegetable crop distributed in over 80 countries. Downy mildew (DM) caused by the obligate oomycete Pseudoperonospora cubensis is especially destructive in cucumber production. So far, few studies on the changes in proteomes during the P. cubensis infection have been performed. Using a newly developed TMT-LC-MS/MS analysis, the proteomes of DM-resistant variety ‘ZJ’ and DM-susceptible variety ‘SDG’ under the P. cubensis infection were investigated. In total, 6400 proteins were identified, 5629 of which were quantified. The differential accumulated proteins (DAPs) exhibited various biological functions and diverse subcellular localizations. KEGG enrichment analysis showed that various metabolic pathways were significantly altered under the P. cubensis infection, such as terpenoid backbone biosynthesis, and selenocompound metabolism in ZJ, and starch and sucrose metabolism in SDG. Most of the enzymes associated with terpenoid backbone synthesis were significantly accumulated in ZJ rather than in SDG, suggesting that pathogen-induced terpenoids accumulation might play an important role in the resistance against P. cubensis infection. Furthermore, a number of pathogenesis-related proteins and heat shock proteins were identified as DAPs, suggesting that DM resistance was controlled by a complex network. Our data allowed us to identify and screen more potential proteins related to the DM resistance.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:13:52.965.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterxiaonan fu
SpeciesList scientific name: Cucumis sativus; NCBI TaxID: 3659;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-23 06:51:28ID requested
12019-11-12 13:45:45announced
22024-10-22 04:13:54announced2024-10-22: Updated project metadata.
Publication List
Zhang P, Zhu Y, Luo X, Zhou S, Comparative proteomic analysis provides insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.). Sci Rep, 9(1):9433(2019) [pubmed]
10.1038/s41598-019-45111-4;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: downy mildew,cucumber, terpenoid backbone biosynthesis, pathogen resistance, differential accumulated protein, Pseudoperonospora cubensis
Contact List
Shengjun Zhou
contact affiliationInstitute of Vegetable, Zhejiang Academy of Agriculture Sciences, Hangzhou, China
contact emailyinxiang0586@sohu.com
lab head
xiaonan fu
contact affiliationzhejiang scientific technology universitiy
contact email2016460649@qq.com
dataset submitter
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Dataset FTP location
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