PXD010844

PXD010844 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis provides novel insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.)
Description	Cucumber (Cucumis sativus L.) is an economically important vegetable crop distributed in over 80 countries. Downy mildew (DM) caused by the obligate oomycete Pseudoperonospora cubensis is especially destructive in cucumber production. So far, few studies on the changes in proteomes during the P. cubensis infection have been performed. Using a newly developed TMT-LC-MS/MS analysis, the proteomes of DM-resistant variety ‘ZJ’ and DM-susceptible variety ‘SDG’ under the P. cubensis infection were investigated. In total, 6400 proteins were identified, 5629 of which were quantified. The differential accumulated proteins (DAPs) exhibited various biological functions and diverse subcellular localizations. KEGG enrichment analysis showed that various metabolic pathways were significantly altered under the P. cubensis infection, such as terpenoid backbone biosynthesis, and selenocompound metabolism in ZJ, and starch and sucrose metabolism in SDG. Most of the enzymes associated with terpenoid backbone synthesis were significantly accumulated in ZJ rather than in SDG, suggesting that pathogen-induced terpenoids accumulation might play an important role in the resistance against P. cubensis infection. Furthermore, a number of pathogenesis-related proteins and heat shock proteins were identified as DAPs, suggesting that DM resistance was controlled by a complex network. Our data allowed us to identify and screen more potential proteins related to the DM resistance.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:13:52.965.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xiaonan fu
SpeciesList	scientific name: Cucumis sativus; NCBI TaxID: 3659;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-23 06:51:28	ID requested
1	2019-11-12 13:45:45	announced
⏵ 2	2024-10-22 04:13:54	announced	2024-10-22: Updated project metadata.

Zhang P, Zhu Y, Luo X, Zhou S, Comparative proteomic analysis provides insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.). Sci Rep, 9(1):9433(2019) [pubmed]

10.1038/s41598-019-45111-4;

curator keyword: Biological, Biomedical

submitter keyword: downy mildew,cucumber, terpenoid backbone biosynthesis, pathogen resistance, differential accumulated protein, Pseudoperonospora cubensis

Shengjun Zhou
contact affiliation	Institute of Vegetable, Zhejiang Academy of Agriculture Sciences, Hangzhou, China
contact email	yinxiang0586@sohu.com
lab head
xiaonan fu
contact affiliation	zhejiang scientific technology universitiy
contact email	2016460649@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD010844

PRIDE project URI

• PRIDE
  1. PXD010844
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis provides novel insights into the complex responses to Pseudoperonospora cubensis infection of cucumber (Cucumis sativus L.)