PXD010835

PXD010835 is an original dataset announced via ProteomeXchange.

Title	A label-free quantitative proteomic analysis reveals amino acids and carbohydrate metabolic enzymes are differentially accumulated in three Jerusalem artichoke (Helianthus tuberosus L.) cultivars exhibiting phenological tuberization characters
Description	Jerusalem artichoke (JA) tubers are an important bio-economy developing crop because of its invaluable bioproducts in both food and biofuel aspects. However, the molecular mechanism of its tuberization, and the differences among different cultivars have been little studied to date. Here, we conducted a comparative proteome profiling of the JA tubers of three different cultivars including PJA, DJA, and HJA, showing phenotypic characteristics. Tuber epidermal pigmentation and underground tuberization habit were different phenological characters in the three cultivars and inulin content was also a physiological character exceptionally DJA regardless of the similar level of total carbohydrate amount. We identified a total of 420 proteins in the tubers and out of 114 showed significantly modulated among the cultivars. GO classification of the DEPs revealed biosynthesis amino acid and carbohydrate metabolic enzymes were differentially expressed in the three cultivar tubers. Integrated physiological inulin content and the biosynthetic protein expression levels among the cultivars suggest that Sucrose:sucrose 1-fructosyltransferase (1-SST) prioritizes inulin biosynthesis rather than rate-limiting enzyme fructan:fructan 1-fructosyltransferases (1-FFT). Furthermore, we confirmed the relationship between transcript-protein expression levels was in discord within inulin biosynthesis enzymes 1-SST and 1-FFT with the terms in previous RT-qPCR results using the same tubers. Our data represent the first report that comparative proteome profiling in JA tubers among the different cultivars and provides the metabolic and molecular basis for understanding carbohydrate metabolism in storage tuber tissue.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_06:53:26.747.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Helianthus tuberosus; NCBI TaxID: 4233;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-21 06:47:53	ID requested
1	2019-05-29 05:41:56	announced
2	2019-07-12 04:31:21	announced	Updated publication reference for DOI(s): 10.1007/s11816-019-00518-3.
⏵ 3	2023-11-14 06:53:27	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Gel-free Proteomics,Inulin, tuber, Jerusalem artichoke (Helianthus tuberosus)

Sun Tae Kim, Hye Sun Cho
contact affiliation	Department of Plant Bioscience, Pusan National University, 1268-50 Samrangjin-ro, Samnangjin-eup, Miryang, 50463, Korea Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Korea.
contact email	stkim71@pusan.ac.kr
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim5505@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/05/PXD010835

PRIDE project URI

• PRIDE
  1. PXD010835
     1. Label: PRIDE project
     2. Name: A label-free quantitative proteomic analysis reveals amino acids and carbohydrate metabolic enzymes are differentially accumulated in three Jerusalem artichoke (Helianthus tuberosus L.) cultivars exhibiting phenological tuberization characters