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PXD010830

PXD010830 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteome of Medusavirus
DescriptionMedusavirus particles was resuspended in PBS containing 0.5% SDS and protease inhibitor cocktail (Nacalai Tesque, Japan) and incubated for 1 h at 65°C. Samples were subjected to trichloroacetic acid (TCA) precipitation, followed by protein quantification using the BCA method. Proteins were reduced for 2 h at 37°C, and subsequently alkylated using iodoacetamide for 30 min at 20 to 25°C (room temperature), followed by treatment with trypsin for 20 h at 37°C. After desalination and concentration, the treated proteins were subjected to LC–MS/MS analysis using EASY-nLC 1200 (Thermo Fisher Scientific Inc., USA) and Q Exactive Plus (Thermo Fisher Scientific Inc., USA).
HostingRepositoryjPOST
AnnounceDate2019-01-30
AnnouncementXMLSubmission_2019-01-29_17:29:16.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGenki Yoshikawa
SpeciesList scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationListunknown modification; L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-21 00:05:50ID requested
12019-01-29 17:29:17announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Medusavirus, purified particles, LC-MS/MS
Contact List
Masaharu Takemura
lab head
Genki Yoshikawa
contact affiliationKyoto University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location