PXD010824

PXD010824 is an original dataset announced via ProteomeXchange.

Title	Characterizing of Haloferax volcanii Hvo_0405 as a Tat substrate
Description	In order to test the hypothesis that Hvo_0405 is a Tat substrate, samples from the membrane and cytoplasm of strains expressing Hvo_0405RR or the mutated Hvo_0405KK were analyzed by mass spectrometry. A semi-enzymatic peptid corresponding to the cleavage of the signal peptide could only be identified in for Hvo_0405RR, indicating that Hvo_0405 is indeed a Tat substrate.
HostingRepository	PRIDE
AnnounceDate	2020-05-11
AnnouncementXML	Submission_2020-07-01_22:31:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Haloferax volcanii (Halobacterium volcanii); NCBI TaxID: 2246;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-20 02:31:35	ID requested
1	2020-05-10 22:30:47	announced
⏵ 2	2020-07-01 22:31:38	announced	2020-07-02: Updated publication reference for PubMed record(s): 32561711, 28069824, 26966177.

Schulze S, Adams Z, Cerletti M, De Castro R, Ferreira-Cerca S, Fufezan C, Gim, é, nez MI, Hippler M, Jevtic Z, Kn, ü, ppel R, Legerme G, Lenz C, Marchfelder A, Maupin-Furlow J, Paggi RA, Pfeiffer F, Poetsch A, Urlaub H, Pohlschroder M, The Archaeal Proteome Project advances knowledge about archaeal cell biology through comprehensive proteomics. Nat Commun, 11(1):3145(2020) [pubmed]

Abdul Halim MF, Stoltzfus JD, Schulze S, Hippler M, Pohlschroder M, ArtA-Dependent Processing of a Tat Substrate Containing a Conserved Tripartite Structure That Is Not Localized at the C Terminus. J Bacteriol, 199(7):(2017) [pubmed]

Esquivel RN, Schulze S, Xu R, Hippler M, Pohlschroder M, Identification of Haloferax volcanii Pilin N-Glycans with Diverse Roles in Pilus Biosynthesis, Adhesion, and Microcolony Formation. J Biol Chem, 291(20):10602-14(2016) [pubmed]

curator keyword: Biological

submitter keyword: Haloferax volcanii, Archaea, Twin-Arginine Translocation, Archaeosortase

Mechthild Pohlschroder
contact affiliation	Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA
contact email	pohlschr@sas.upenn.edu
lab head
Stefan Schulze
contact affiliation	University of Pennsylvania
contact email	sschulze@sas.upenn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD010824

PRIDE project URI

• PRIDE
  1. PXD010824
     1. Label: PRIDE project
     2. Name: Characterizing of Haloferax volcanii Hvo_0405 as a Tat substrate