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PXD010816

PXD010816 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRelease of the plant peptide hormone PEP1 from its precursor PROPEP1 in response to physical damage is due to Ca2+-dependent activation of METACASPASE4.
DescriptionPhysical damage to cells leads to the release of immunomodulatory peptides in order to elicit wound defense responses in the surrounding tissue. In Arabidopsis thaliana, the intracellular PROPEP1 protein precursor is processed into the mature 2.5 kDa PEP1 before release. However, the maturation mechanism remained unknown. Here, we demonstrate that both at tissue level and upon highly precise single-cell damage by multiphoton laser wounding, the cysteine protease METACASPASE4 is instantly activated in a spatiotemporal Ca2+ dependent manner, and is necessary and sufficient for PEP1 maturation. Our results reveal a robust and most likely conserved mechanism that links the intracellular and calcium dependent activation of a specific cysteine protease with the maturation of damage induced wound defense signals. Peptide coverage was obtained for the PROPEP1-YFP-HA fusion protein used in this study and its lower molecular weight processed forms (derived from in vivo proteolysis). In gel digest of immunoprecipitated bands was subjected to mass spectrometry analysis, repeated twice on different mass spectrometers (Velos and Q-Exactive HF).
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:10:56.840.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD010816
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPatrick Willems
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListPropionamide; Glu->pyro-Glu; Oxidation; Acetyl; Gln->pyro-Glu
InstrumentLTQ Orbitrap Velos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-20 01:41:25ID requested
12019-04-18 05:16:43announced
22024-10-22 04:11:00announced2024-10-22: Updated project metadata.
Publication List
Hander T, Fern, á, ndez-Fern, á, ndez Á D, Kumpf RP, Willems P, Schatowitz H, Rombaut D, Staes A, Nolf J, Pottie R, Yao P, Gon, ç, alves A, Pavie B, Boller T, Gevaert K, Van Breusegem F, Bartels S, Stael S, -dependent metacaspases for release of immunomodulatory peptides. Science, 363(6433):(2019) [pubmed]
10.6019/PXD010816;
10.1126/science.aar7486;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Arabidopsis, protease,METACASPASE4, proteolysis, PROPEP, physical damage
Contact List
Frank Van Breusegem
contact affiliationDepartment of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium VIB-UGent Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
contact emailfrbre@psb.ugent.be
lab head
Patrick Willems
contact affiliationPSB-VIB
contact emailpawil@psb.ugent.be
dataset submitter
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Dataset FTP location
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