PXD010816 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Release of the plant peptide hormone PEP1 from its precursor PROPEP1 in response to physical damage is due to Ca2+-dependent activation of METACASPASE4. |
Description | Physical damage to cells leads to the release of immunomodulatory peptides in order to elicit wound defense responses in the surrounding tissue. In Arabidopsis thaliana, the intracellular PROPEP1 protein precursor is processed into the mature 2.5 kDa PEP1 before release. However, the maturation mechanism remained unknown. Here, we demonstrate that both at tissue level and upon highly precise single-cell damage by multiphoton laser wounding, the cysteine protease METACASPASE4 is instantly activated in a spatiotemporal Ca2+ dependent manner, and is necessary and sufficient for PEP1 maturation. Our results reveal a robust and most likely conserved mechanism that links the intracellular and calcium dependent activation of a specific cysteine protease with the maturation of damage induced wound defense signals. Peptide coverage was obtained for the PROPEP1-YFP-HA fusion protein used in this study and its lower molecular weight processed forms (derived from in vivo proteolysis). In gel digest of immunoprecipitated bands was subjected to mass spectrometry analysis, repeated twice on different mass spectrometers (Velos and Q-Exactive HF). |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:10:56.840.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD010816 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Patrick Willems |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | Propionamide; Glu->pyro-Glu; Oxidation; Acetyl; Gln->pyro-Glu |
Instrument | LTQ Orbitrap Velos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-20 01:41:25 | ID requested | |
1 | 2019-04-18 05:16:43 | announced | |
⏵ 2 | 2024-10-22 04:11:00 | announced | 2024-10-22: Updated project metadata. |
Publication List
Hander T, Fern, á, ndez-Fern, á, ndez Á D, Kumpf RP, Willems P, Schatowitz H, Rombaut D, Staes A, Nolf J, Pottie R, Yao P, Gon, ç, alves A, Pavie B, Boller T, Gevaert K, Van Breusegem F, Bartels S, Stael S, -dependent metacaspases for release of immunomodulatory peptides. Science, 363(6433):(2019) [pubmed] |
10.6019/PXD010816; |
10.1126/science.aar7486; |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Arabidopsis, protease,METACASPASE4, proteolysis, PROPEP, physical damage |
Contact List
Frank Van Breusegem |
contact affiliation | Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium VIB-UGent Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium |
contact email | frbre@psb.ugent.be |
lab head | |
Patrick Willems |
contact affiliation | PSB-VIB |
contact email | pawil@psb.ugent.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010816
- Label: PRIDE project
- Name: Release of the plant peptide hormone PEP1 from its precursor PROPEP1 in response to physical damage is due to Ca2+-dependent activation of METACASPASE4.