PXD010816

PXD010816 is an original dataset announced via ProteomeXchange.

Title	Release of the plant peptide hormone PEP1 from its precursor PROPEP1 in response to physical damage is due to Ca2+-dependent activation of METACASPASE4.
Description	Physical damage to cells leads to the release of immunomodulatory peptides in order to elicit wound defense responses in the surrounding tissue. In Arabidopsis thaliana, the intracellular PROPEP1 protein precursor is processed into the mature 2.5 kDa PEP1 before release. However, the maturation mechanism remained unknown. Here, we demonstrate that both at tissue level and upon highly precise single-cell damage by multiphoton laser wounding, the cysteine protease METACASPASE4 is instantly activated in a spatiotemporal Ca2+ dependent manner, and is necessary and sufficient for PEP1 maturation. Our results reveal a robust and most likely conserved mechanism that links the intracellular and calcium dependent activation of a specific cysteine protease with the maturation of damage induced wound defense signals. Peptide coverage was obtained for the PROPEP1-YFP-HA fusion protein used in this study and its lower molecular weight processed forms (derived from in vivo proteolysis). In gel digest of immunoprecipitated bands was subjected to mass spectrometry analysis, repeated twice on different mass spectrometers (Velos and Q-Exactive HF).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:10:56.840.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD010816
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Patrick Willems
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	Propionamide; Glu->pyro-Glu; Oxidation; Acetyl; Gln->pyro-Glu
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-20 01:41:25	ID requested
1	2019-04-18 05:16:43	announced
⏵ 2	2024-10-22 04:11:00	announced	2024-10-22: Updated project metadata.

Hander T, Fern, á, ndez-Fern, á, ndez Á D, Kumpf RP, Willems P, Schatowitz H, Rombaut D, Staes A, Nolf J, Pottie R, Yao P, Gon, ç, alves A, Pavie B, Boller T, Gevaert K, Van Breusegem F, Bartels S, Stael S, -dependent metacaspases for release of immunomodulatory peptides. Science, 363(6433):(2019) [pubmed]

10.6019/PXD010816;

10.1126/science.aar7486;

curator keyword: Biological, Biomedical

submitter keyword: Arabidopsis, protease,METACASPASE4, proteolysis, PROPEP, physical damage

Frank Van Breusegem
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium VIB-UGent Center for Plant Systems Biology, VIB, 9052 Ghent, Belgium
contact email	frbre@psb.ugent.be
lab head
Patrick Willems
contact affiliation	PSB-VIB
contact email	pawil@psb.ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD010816
     1. Label: PRIDE project
     2. Name: Release of the plant peptide hormone PEP1 from its precursor PROPEP1 in response to physical damage is due to Ca2+-dependent activation of METACASPASE4.