PXD010811 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The majority of viral peptides displayed on class I MHC are immunogenic during infection |
Description | CD8+ T cells are essential effectors in anti-viral immunity, recognising short virus-derived peptides presented by MHC class I (pMHCI) on the surface of infected cells. However, the fraction of viral pMHCI on infected cells that are immunogenic has not been shown for any virus. To approach this fundamental question, we used peptide sequencing by high-resolution mass spectrometry to identify nearly 200 vaccinia virus pMHCI presented on infected mouse cells. Next, we screened each peptide for immunogenicity in multiple virus-infected mice, revealing a wide range of immunogenicities. A surprisingly high fraction (>80%) of pMHCI were immunogenic at least one infected animal and around half of these were dominant, which we define as being immunogenic across more than half of the mice screened. The high number of peptides found to be immunogenic and the variability in immunogenicity across mice give us new insight into the specificity of anti-viral CD8+ T cell responses. |
HostingRepository | PRIDE |
AnnounceDate | 2019-01-23 |
AnnouncementXML | Submission_2019-02-11_04:56:05.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nathan Croft |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Vaccinia virus WR; NCBI TaxID: 10254; |
ModificationList | amidated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-17 06:51:58 | ID requested | |
1 | 2019-01-23 01:18:25 | announced | |
⏵ 2 | 2019-02-11 04:56:06 | announced | Updated publication reference for PubMed record(s): 30718433. |
Publication List
Croft NP, Smith SA, Pickering J, Sidney J, Peters B, Faridi P, Witney MJ, Sebastian P, Flesch IEA, Heading SL, Sette A, La Gruta NL, Purcell AW, Tscharke DC, Most viral peptides displayed by class I MHC on infected cells are immunogenic. Proc Natl Acad Sci U S A, 116(8):3112-3117(2019) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Mouse, LC-MS/MS, vaccinia virus |
Contact List
Anthony Purcell |
contact affiliation | Infection and Immunity Program, Biomedicine Discovery Institute & Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia |
contact email | anthony.purcell@monash.edu |
lab head | |
Nathan Croft |
contact affiliation | Monash University |
contact email | nathan.croft@monash.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD010811 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010811
- Label: PRIDE project
- Name: The majority of viral peptides displayed on class I MHC are immunogenic during infection