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PXD010811

PXD010811 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe majority of viral peptides displayed on class I MHC are immunogenic during infection
DescriptionCD8+ T cells are essential effectors in anti-viral immunity, recognising short virus-derived peptides presented by MHC class I (pMHCI) on the surface of infected cells. However, the fraction of viral pMHCI on infected cells that are immunogenic has not been shown for any virus. To approach this fundamental question, we used peptide sequencing by high-resolution mass spectrometry to identify nearly 200 vaccinia virus pMHCI presented on infected mouse cells. Next, we screened each peptide for immunogenicity in multiple virus-infected mice, revealing a wide range of immunogenicities. A surprisingly high fraction (>80%) of pMHCI were immunogenic at least one infected animal and around half of these were dominant, which we define as being immunogenic across more than half of the mice screened. The high number of peptides found to be immunogenic and the variability in immunogenicity across mice give us new insight into the specificity of anti-viral CD8+ T cell responses.
HostingRepositoryPRIDE
AnnounceDate2019-01-23
AnnouncementXMLSubmission_2019-02-11_04:56:05.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNathan Croft
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Vaccinia virus WR; NCBI TaxID: 10254;
ModificationListamidated residue; phosphorylated residue; monohydroxylated residue; acetylated residue; deamidated residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02018-08-17 06:51:58ID requested
12019-01-23 01:18:25announced
22019-02-11 04:56:06announcedUpdated publication reference for PubMed record(s): 30718433.
Publication List
Croft NP, Smith SA, Pickering J, Sidney J, Peters B, Faridi P, Witney MJ, Sebastian P, Flesch IEA, Heading SL, Sette A, La Gruta NL, Purcell AW, Tscharke DC, Most viral peptides displayed by class I MHC on infected cells are immunogenic. Proc Natl Acad Sci U S A, 116(8):3112-3117(2019) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Mouse, LC-MS/MS, vaccinia virus
Contact List
Anthony Purcell
contact affiliationInfection and Immunity Program, Biomedicine Discovery Institute & Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia
contact emailanthony.purcell@monash.edu
lab head
Nathan Croft
contact affiliationMonash University
contact emailnathan.croft@monash.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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