PXD010808 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | B cell Lymphomas present Immunoglobulin Neoantigens |
Description | Immunoglobulin gene rearrangement and somatic hypermutation have the potential to create neoantigens in non-Hodgkin B cell lymphoma. However, the presentation of these putative immunoglobulin neoantigens by B cell lymphomas has not been proven. We used MHC immunoprecipitation followed by liquid chromatography and tandem mass spectrometry (LC-MS/MS) to define antigens presented by follicular lymphomas (FL), chronic lymphocytic leukemias (CLL), diffuse large B cell lymphoma (DLBCL) and mantle cell lymphomas (MCL). We found presentation of the clonal immunoglobulin molecule, including neoantigens by both class I and class II MHC, though more commonly in class II MHC. To determine whether B cell activation could promote presentation of immunoglobulin neoantigens, we used a toll-like receptor 9 (TLR9) agonists to upregulate expression of MHC-II. This resulted in enhanced class II MHC presentation of the immunoglobulin variable region including neoantigens. These findings demonstrate that immunoglobulin neoantigens are presented across most subtypes of B cell lymphomas. Activation of lymphoma cells to upregulate antigen presentation boosts presentation of immunoglobulin neoantigens and represents a strategy for augmenting lymphoma immunotherapies. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:11:03.495.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Niclas Olsson |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | cysteinylation (disulfide with free L-cysteine); phosphorylated residue; monohydroxylated residue; deamidated residue |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2018-08-17 06:47:24 | ID requested | |
1 | 2019-01-07 05:15:42 | announced | |
⏵ 2 | 2024-10-22 04:11:04 | announced | 2024-10-22: Updated project metadata. |
Publication List
Khodadoust MS, Olsson N, Chen B, Sworder B, Shree T, Liu CL, Zhang L, Czerwinski DK, Davis MM, Levy R, Elias JE, Alizadeh AA, B-cell lymphomas present immunoglobulin neoantigens. Blood, 133(8):878-881(2019) [pubmed] |
10.1182/blood-2018-06-845156; |
Keyword List
curator keyword: Biological |
submitter keyword: lymphoma, neoantigens,MHC, Orbitrap |
Contact List
Joshua E Elias |
contact affiliation | Department of Chemical & Systems Biology, Stanford University, USA |
contact email | josh.elias@stanford.edu |
lab head | |
Niclas Olsson |
contact affiliation | Calico Life Sciences |
contact email | nic.e.olsson@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD010808
- Label: PRIDE project
- Name: B cell Lymphomas present Immunoglobulin Neoantigens