PXD010802

PXD010802 is an original dataset announced via ProteomeXchange.

Title	Proteomic consequences of early postnatal, chronic THC exposure for the adolescent and adult hippocampus
Description	This study aims to identify the proteomic targets of THC in the early postnatal hippocampus of developing mice. Therefore, early postnatal C57Bl/6 mice (P5) were exposed daily and for 30 days to plant extracted THC (either 1 mg/kg or 5 mg/kg) or vehicle solution (saline with 3% Tween® 80) for the control group. All animals stayed with their mothers until P25 and after the initial drug exposure time (until P35) animals were given a drug-free resting period. The animals were sacrificed, and their hippocampus was dissected and prepared for the following proteomic analysis at either P48 or after an extended resting period at P120. We found 31 proteins to be changed after THC exposure compared to vehicle at P48 and 186 proteins showing modifications at P120. Gene ontology classification of protein targets revealed a substantial amount of proteins involved in metabolic processes of neurons after THC exposure. The results highlight the vulnerability of the developing hippocampus towards THC exposure and identify the mitochondrial as well as other cell metabolic processes as potential drug targets.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:14:46.704.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Edit Szodorai
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	L-cysteine methyl disulfide; iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-16 07:49:51	ID requested
1	2020-11-09 08:11:50	announced
⏵ 2	2024-10-22 05:14:47	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological, Biomedical

submitter keyword: hippocampus, mouse brain development,THC

Univ. Prof. Dr. Tibor Harkany
contact affiliation	1 Department of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, Spitalgasse 4, A-1090 Vienna, Austria; 2 Department of Neuroscience, Karolinska Institutet, Retzius väg 8, SE-17177 Stockholm, Sweden
contact email	tibor.harkany@meduniwien.ac.at
lab head
Edit Szodorai
contact affiliation	Dept. of Molecular Neurosciences, Center for Brain Research, Medical University of Vienna, Vienna, Austria
contact email	edit.szodorai@meduniwien.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/11/PXD010802

PRIDE project URI

• PRIDE
  1. PXD010802
     1. Label: PRIDE project
     2. Name: Proteomic consequences of early postnatal, chronic THC exposure for the adolescent and adult hippocampus