PXD010781

PXD010781 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics of immunocaptured mitochondria from Astrocytes
Description	Mitochondria vary in morphology and function in different tissues, however little is known about their molecular diversity among cell types. To investigate mitochondrial diversity in vivo, we developed an efficient protocol to isolate cell type-specific mitochondria based on a new MitoTag mouse. We profiled the mitochondrial proteome of three major neural cell types in cerebellum and identified a substantial number of differential mitochondrial markers for these cell types in mice and humans. Based on predictions from these proteomes, we demonstrate that astrocytic mitochondria metabolize long-chain fatty acids more efficiently than neurons. Moreover, we identified Rmdn3 as a major determinant of ER-mitochondria proximity in Purkinje cells. Our novel approach enables exploring mitochondrial diversity on the functional and molecular level in many in vivo contexts.
HostingRepository	PRIDE
AnnounceDate	2019-09-03
AnnouncementXML	Submission_2019-09-03_02:19:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2018-08-15 03:13:37	ID requested
⏵ 1	2019-09-03 02:19:24	announced

Dataset with its publication pending

ProteomeXchange project tag: Mitochondria (HPP)

curator keyword: Biological

submitter keyword: Astrocytes, Mitochondria

Thomas Misgeld
contact affiliation	Institute of Neuronal Cell Biology Technical University of Munich Germany
contact email	thomas.misgeld@tum.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD010781

PRIDE project URI

• PRIDE
  1. PXD010781
     1. Label: PRIDE project
     2. Name: Quantitative proteomics of immunocaptured mitochondria from Astrocytes